【作者】 靳恒； 关瑞章； 李忠琴； 罗鸣钟；

【Author】 Jin Heng,Guan Rui-zhang*,Li Zhong-qin,Luo Ming-zhong(Fisheries College of Jimei University,Engineering Research Center of the Modern Industrial Technology for Eel,Ministry of Education,Xiamen 361021,China)

【机构】 集美大学水产学院鳗鲡现代产业技术教育部工程研究中心；

【摘要】 本实验采用正交试验优化超声辅助提取黄芩中总黄酮,并应用高速逆流色谱对粗提物进行分离纯化,得到纯度较高的总黄酮有效部位。结果显示最佳超声提取工艺条件为:70%浓度乙醇、超声功率300W、超声时间20min、料液比1:30,粗提物总黄酮含量2.81%。高速逆流色谱两相溶剂系统为乙酸乙酯-甲醇-水-醋酸(5:0.5:4.5:0.05,V/V)。分析型高速逆流色谱进样1.5mg,流动相流速0.5mg/ml,转速1800r/min,检测波长278nm,最后由谱图可知分离得到6种黄酮类物质。本实验应用乙醇超声提取黄芩总黄酮,提高了有效部位的提取率,缩短了提取时间。采用分析型高速逆流色谱摸索分离所需的溶剂系统,为后期制备型高速逆流色谱提供条件。分离的有效部位将被用来测定对常见水产致病菌的抑菌效果。更多还原

【Abstract】 To optimize the ultrasound-assisted extraction of total flavonoids from Radix Scutellariae with orthogonal test design,and high-speed counter-current chromatography(HSCCC) was applied for the separation and purification of flavonoids.The results showed that the optimal condition of extraction was: extraction with 70% ethanol,ultrasounic power 300W,extracting time 20min,ratio of solid to liquid 1:30,the average yield of total flavonoids was 2.81%.The experiment of HSCCC was performed with a two-phase solvent system composed of ethyl acetate-methanol-water-acetic acid(5:0.5:4.5:0.05,V/V),the detection wavelength was detected at 278nm.By the analytical type HSCCC crude extract was sampled 1.5mg,under the conditions of a flow rate of 0.05mL/min,1800r/min and the detection wavelength of 278nm.Finally we got six kinds of flavonoids from the spectrum diagram.This experiment applied ethanol ultrasound-assisted extraction of total flavonoids from Radix Scutellariae,it can improve the the extraction efficiency of the effective parts and shorten the extraction time.Try to find the solvent system by the analytical type HSCCC,to preaper the condition of the preparative HSCCC.The effective parts which were got by the preparative HSCCC will be tested the susceptibility against the pathogenic bacteria from the aquatic animals.更多还原