A genetically incorporated crosslinker reveals outer membrane protein quality control mechanims in acid resistance

【作者】 张萌； 林世贤； 宋新文； 昌增益； 陈鹏；

【机构】 北京大学化学与分子工程学院； 北京大学生命科学学院；

【摘要】 <正>Acid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach(pH 1-3).The client pro-teins of these chaperones and the protecting mechanisms remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions.In previous research,we have reported a newly developed ge-netically encoded,highly efficient protein photocrosslinking probe,which enabled us to profile the in vivo substrates of a major acid-protection chaperone,HdeA,in Escherichia coli periplasm.It helps to reveal a更多还原