【作者】 张业鹏； 张国文；

【Author】 ZHANG Ye-peng,ZHANG Guo-wen~* State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China

【机构】 南昌大学食品科学与技术国家重点实验室；

【摘要】 在生理条件下(pH 7.4),采用荧光光谱、紫外可见光谱、圆二色谱(CD)和傅里叶红外光谱(FTIR)技术,研究了除草剂氰草津与小牛胸腺DNA(ctDNA)相互作用。结果表明,氰草津以嵌插方式与ctDNA结合,这种结合导致ctDNA由B构型部分地向A构型转变,并伴随一定程度地聚合,测得的热力学参数表明,疏水作用和氢键是二者结合的主要驱动力。更多还原

【Abstract】 The interaction of the herbicide(cyanazine) with calf thymus DNA(ctDNA) was investigated by fluorescence,UV-Vis absorption,circle dichroism(CD) and Fourier transform infrared(FTIR) spectroscopy in physiological buffer solution(pH 7.4).The results indicated that cyanazine could bind to calf thymus DNA by the intercalation mode.B-like DNA structure transforms to an A-like conformation accompanying with some aggregatioa The calculated thermodynamic parameters indicated that hydrophobic interaction and hydrogen bonds played a main role in the binding of cyanazine to ctDNA.更多还原