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莱氏野村菌对甜菜夜蛾及斜纹夜蛾的毒力测定
THE VIRULENCE OF NOMURAEA RILEYI ON THE LARVAE OF NOCTUID PESTS,LAPHYGMA EXIGUA AND SPODOPTERA LITURA
【Author】 Xie Ming~1 Wan Fang-hao~1 Li Feng~1 Guan Xiong~2 Qiu Wei-liang~1 1 Institute of Biological Control,Chinese Acadamy of Agricultural Sciences,Beijing 100081 2 Fujian University of Agriculture and Forestry,Fuzhou 350002
【机构】 中国农业科学院生物防治研究所; 福建农林大学;
【摘要】 本研究采用常规的浸蘸法测定莱氏野村菌菌株CA22的毒力。实验结果显示,该菌株对甜菜夜蛾、斜纹夜蛾均有很高的毒力,致死中浓度(LC50)随虫龄的增大而增大,对甜菜夜蛾二龄幼虫的LCs0值为0.65×107孢子/ml,对五龄幼虫的LC50值为9.16×107孢子/ml。甜菜夜蛾低龄幼虫对该菌的敏感性比高龄幼虫大14倍。该菌株对斜纹夜蛾二、三、四龄幼虫的LC50分别为7.68×107孢子/ml、7.81×107孢子/ml、7.46×107孢子/ml,它们之间的差异不显著;但对五龄幼虫的LC50较高,27.1×107孢子/ml。斜纹夜蛾低龄幼虫对该菌的敏感性比高龄幼虫大3.5倍。
【Abstract】 This bioassay uses liquid immersion method to test the virulence of isolate CA22 of Nomuraea rileyi from Fujian province.The results showed that the isolate have high toxicity to the noctuid pests,Laphygma exigua,Spodoptera litura.The LC50 rises along with the instar increase.The LC50 of 2nd instar of Laphygma exigua is 0.65×107 conidia/ml,and of 5th instar of Laphygma exigua is 9.16×107 conidia/ml.The sensitivity of lower instar of Laphygma exigua to this isolate is 14 times as many as those of last instar.The LC50 volume of this isolate to 2nd instar,3rd instar,4th instar of Spodoptera litura are 7.68×107 conidia/ml,7.81×107 conidia/ml and 7.46×107 conidia/ml respectively and there are no statistic significant.While the LC50 volume to 5th instar of Spodoptera litura is 27.1×107 conidia/ml,which is significantly higher(ca 3.5 times) than the other instars.
【Key words】 Nomuraea rileyi; Laphygma exigua; Spodoptera litura; virulence;
- 【会议录名称】 《中国虫生真菌研究与应用》(第五卷)
- 【会议时间】2003-04-01
- 【分类号】S476.1