【作者】 汤坚； 王成树； 李农昌； 李增智； 樊美珍；

【Author】 Tang Jian (General Station of Forest Pest Control,Anhui,Hefei,230031) Wang Chengshu Li Nongchang Li Zengzhi Fan Meizhen (Auhui Agricultural University,Hefei 230036)

【机构】 安徽省森林病虫防治总站； 安徽农业大学；

【摘要】 本研究首先通过相容性试验,筛选出球孢白僵菌（Beauveria bassiana,下称白僵菌）混合粉剂加工的适宜载体、紫外保护剂及灭幼脲Ⅲ号原粉作为增效剂。通过正交设计（L₄³正交表）,得到白僵菌混合粉剂的最佳配方,其组成为:20%的紫外保护剂OF,4%的灭幼脲Ⅲ号原粉,25%的凹凸棒土及51%的孢子粉。产品经充氮包装,室温下贮藏半年后的萌发率为70.56%。白僵菌混合粉剂主要技术指标为:含孢量大于500×10⁸/g,含水量小于10%,贮藏一年后的萌发率大于70%,对3～4龄马尾松毛虫毒力生物测定的LT₅₀为5～7d,灭幼脲含量4%～4.5%,对大白鼠的经口毒性>5000mg/kg。产品的每公顷用量为300～300.75g,使用成本为13.5～16.9元/hm²。室内生物测定,混合粉剂对3～4龄马尾松毛虫幼虫和LT₅₀为5.7d。林间放菌,防治效果达74.2%。混合粉剂处理对天敌没有影响,处理区和空白对照区,寄生性天敌致死率没有显著差异。更多还原

【Abstract】 After the compatibility of the conidia with the a vailable fillers being tested,UV protectant and the diflubenzuron,the formulae of the Beauveria mixed dust were determined by L4³ orthogonal design.The combination of the every formulae was 20%OF UV-protectant,4% stock powder of dif lubenzuron,25%carrier of attapulgite and 51%conidial powder.The germination rates of the conidia,formulated and then charged with nitrogen,were 70.56%after stored for half of a year.The main technological parameters of the mixed dust were given as follow;conidial count>500 X 108/g,water content< 10%,germination rate>70%after stored at normal condition for one year,the LT₅₀ to the larvae of 3—4 instar pine caterpillars within 5～7 days,the content of diflubenzuron within 4%～4.5%and the toxicity to adult mouse>5000mg/kg.The application cost reached 13.5～16.9 yuan/ha while 300～375 germ of dust was sprayed per hectare.The bioassay result showed the LT₅₀ of the dust to the thirdfourth instar larvae of Masson’ pine caterpillars was 5.7 days.The control efficacy of the dust against the larvae of Masson’s pine caterpoliars reached 74.27%while the unformulated conidia did 55.13%in the contrast.The results also showed that there was on negetive effect on caterpillars’natural enimies,they jointly killed the pests.更多还原