【作者】 吴杰； 高雄伟； 郑金荣； 吴欢； 潘晶晶；

【Author】 Department of Neurosurgery,Wenling Second People’s Hospital, Wenling Zhejiang 317502,China; WU Jie GAO xiong-wei1 ZhENG jin-rong1 WU huan1 Department of Neurosurgery,Wuhu Second People’s Hospital,Wuhu 241000,China. PAN jing-jing

【机构】 浙江省温岭市第二人民医院神经外科； 安徽省芜湖市第二人民医院神经外科；

【摘要】 目的观察骨髓基质细胞(BMSCs)经颈内动脉移植对短暂脑缺血大鼠的行为学及脑皮层组织血管内皮生长因子(VEGF)表达的影响。探讨BMSCs经颈内动脉移植对短暂脑缺血大鼠的作用及其可能机制。方法短暂脑缺血模型的29只SD大鼠被随机分为4组:PET观察组(n=5)、治疗组(n=8)、对照组(n=8)、假手术组(n=8)。18F-FDG标记的BMSCs经右侧颈内动脉注射入颅,用小动物PET观察移植后3小时BMSCs在体内的分布。用Morris水迷宫实验比较各组动物行为学差异,免疫组化法检测各组动物脑皮层组织VEGF阳性细胞数的差异。结果 BMSCs经右侧颈内动脉注射入颅后3小时,细胞主要分布于双侧颅内,右侧多余左侧;Morris水迷宫实验中对照组和假手术组与移植组相比,潜伏期延长,穿越原有平台次数减少,差异有统计学意义(P<0.05)。HE染色观察到移植组神经元缺失减少,微血管增生明显。移植组在皮层脑组织和海马脑组织的VEGF阳性细胞数高于对照组及假手术组,差异有统计学意义(P<0.05)。结论 BMSCs经动脉移植可以减少脑缺血大鼠神经细胞的丢失,促进脑组织VEGF的表达和血管增生,改善学习和记忆功能。以18F-FDG标记BMSCs,用PET观察,可以用于细胞移植的早期活体示踪研究。更多还原

【Abstract】 Objective To observe the effects of bone marrow stromal cell(BMSCs) transplantation on expression of vascular endochelial growth factor(VEGF) and outcome in rats with transient ischemia by internal carotid artery(ICA),and explore the effects and mechanism of treatment.Method 29 Rats were subjected to transient ischemia and 5 of them were transplanted with BMSCs labeled with 18F-FDG by ICA, then traced by minor PET.The rest 24 rats were randomly divided into treatment group(n=8),control group (n=8) and Sham-operation control group(n=8).treatment group were injected with BMSCs,control group with PBS and Sham-operation control group deal with no special treatment.The rats were humanely killed 7 days later.Behavioral tests and histochemical and immunohistochemical staining were performed.Results Minor PET scanning showed that 3 hours after BMSCs transplantation,the donor cells were mainly found in brains of recipient rats.Morris water maze test results showed the escape latency was longer and total swimming times in platform quadrant as well as crossing times were less in control group and Sham-operation control group than in treatment group(P<0.05).BMSCs significantly reduced neuronal loss and promoted microvascular proliferation.The number of VEGF positive cells in the rats brain’s cortex and hippocampus in treatment group was significantly higher than the other two groups(P<0.05).Conclusions Intraarterial administration of BMSCs can reduce neuronal loss,upregulate VEGF expression,promote Vascular proliferation and improve functional outcome.18F-FDG can be used to label BMSCs in vitro and PET can be applied to track BMSCs labeled with 18F-FDG in vivo after transplantation.更多还原