【作者】 孙慧娟； 吕杨超； 胡剑锋； 赵永亮；

【机构】 内蒙古大学化学化工学院； 内蒙古疾病预防控制中心； 呼和浩特市环境监测中心站；

【摘要】 本文研究了稀土配合物与DNA的作用方式。分别合成了以La、Nd、Tb、Ho、Tm、Yb、Y为中心,以肉桂酸（Hcin）和邻菲啰啉（phen）为配体的七种三元稀土固态配合物。经C、N、H元素分析、稀土络合滴定、热稳定性分析、红外光谱和紫外光谱分析,推测三元配合物的组成为RE（cin）₃phen(RE³⁺=La³⁺、Nd³⁺、Tb³⁺、Ho³⁺、Tm³⁺、Yb³⁺、Y³⁺、配合物中RE³⁺离子与肉桂酸的羧基氧原子及邻菲啰啉的两个氮原子配位成键。采用紫外光谱法及黏度法研究了稀土三元配合物与小牛胸腺DNA（CT-DNA）的作用。测试结果表明随着CT-DNA浓度的增加,配合物的最大吸收峰发生红移,且最大吸收峰位的吸光度值逐渐减少;粘度测试表明,随着配合物的加入,CT-DNA的相对黏度逐渐增大。这些结果表明配合物是以插入方式与CT-DNA结合,配合物中的平面分子phen在一定程度上插入到了CT-DNA的碱基对中。更多还原

【Abstract】 Interaction of rare earth complexes and DNA was studied in this paper.Seven complexes of rare earth were synthesized with cinnamic acid（Hcin） as the first ligand and 1,10-Phenanthroline as the second ligand.The chemical formula of the complexes was RE（cin）³phen(RE³⁺= La³⁺、Nd³⁺、Tb³⁺、Ho³⁺、Tm³⁺、Yb³⁺、Y³⁺),they were characterized by elemental analysis and rare earth coordination titration.IR spectra and U V spectra have been studied and the results indicated that the rare earth ions are bounded with the carboxyl oxygen atoms of cinnamic acid,and with the N atoms of 1,10-Phenanthroline.The actions of the rare earth complexes and CT-DNA with UV spectra were researched.When the concentration of CT-DNA is increased,the largest absorption peak is gradually red shift and also weak,which showed that the interaction modes of complexes and CT-DNA are intercalation.The interactions of part complexes with CT-DNA have been investigated by viscosity experiments.The results suggested that the complexes bound to CT-DNA by intercalation.Fluorescence quenching constants were obtained according to the classical Stern?Volmer quenching formula,which made a comparison of binding capacity between CT-DNA and complexes.更多还原