【作者】 田文霞； 李家奎； 覃平； 王瑞； 宁官保； 乔建钢； 李宏全； 毕丁仁； 潘思轶； 郭定宗；

【Author】 TIAN Wen-xia1,2,3,LI Jia-kui1,QIN Ping1,Wang Rui1,Ning Guan-bao2,Qiao Jian-gang2,Li Hong-quan2,BI Ding-ren 4,Pan Si-yi3,Guo Ding-zong1,~* (1 College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,430070,China; 2College of Veterinary Medicine,Shanxi Agricultural University,Taigu Shanxi 030801,China; 3College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070, China;and 4State Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine, Huazhong Agricultural University,Wuhan 430070,China)

【机构】 华中农业大学动物医学院； 山西农业大学动物科技学院； 华中农业大学食品科技学院； 华中农业大学动物医学院农业微生物学国家重点实验室；

【摘要】 【目的】研究肉鸡胫骨软骨发育不良早期钙黏蛋白1(CDH1)的差异表达。【方法】在试验第1、2、6天,对试验鸡只进行剖杀,速采取胫骨生长板放入4%多聚甲醛溶液于4℃固定,做CDH1免疫组化分析;提取对照组和饲喂福美双组的生长板总RNA,采用real-time PCR对CDH1基因进行差异表达验证。【结果】钙黏蛋白1(CDH1)基因上调表达与芯片结果一致。CDH1/E-cadherin的定位和表达分析显示,主要在前肥大、肥大区软骨细胞表达,增殖区软骨细胞无表达,钙化区软骨细胞表达少,呈弥散状分布于细胞浆中,而且在饲喂福美双组呈上调表达,与定量PCR变化结果相一致。【结论】为认识肉鸡TD的病因、从分子水平阐明其发病机制提供了可靠的理论依据。更多还原

【Abstract】 【Objective】The aim of this study was to study the differential expression of cadherin 1(CDH1) in the growth plate of broiler chickens with tibial dyschondroplasia(TD) induced by thiram at the early stage.【Method】Chicks were dissected and growth plates were fixed in 4%paraform respectively in 4℃at d 1,2 and 6.RNA was extracted from the growth plates of control and thiram-fed Chickens.Then differential expression of CDH1 was identified by real-time PCR and immunohistology.【Result】The results showed that the expression of cadherin 1 (CDH1) was up-regulated,which corresponded to the results by microarray.CDH1 protein synthesis was mainly localized in cytoplasm the prehypertrophic and hypertrophic chondrocyte of control or TD growth plates. Noticeably increased CDH1 was detected in TD growth plates on days 1,2 and 6 after feeding thiram diet,which mirrored with their mRNA differential expression,and highly increased CDH1 staining is clearly seen at days 2 and 6 especially.【Conclusion】Our findings may provide reliable molecular cytology basis on understanding the molecular pathogenesis of TD.更多还原