【作者】 李荣芳； 邬静； 袁莉芸； 文利新； 袁慧；

【Author】 LI Rong-fang,WU Jing,YUAN Li-yun,WEN Li-xin,YUAN Hui~* (College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China)

【机构】 湖南农业大学动物医学院；

【摘要】 【研究目的】通过氯丙嗪染毒体外培养的大鼠卵巢颗粒细胞,研究其对卵巢颗粒细胞的毒性作用,并初步探讨可能的作用机制。【方法】对未成熟的wistar大鼠卵巢颗粒细胞进行原代培养,用不同浓度的氯丙嗪(0、0.1、1、10μM)染毒24h。MTT法检测细胞相对活力,Hoechst 33258检测颗粒细胞的凋亡变化,RT-PCR检测凋亡调控基因Bax、Bcl-2和P53 mRNA的表达。【结果】在本次实验所设置的剂量范围内,与对照组比较,氯丙嗪能显著地促进颗粒细胞凋亡(P<0.01),呈浓度依赖关系;RT-PCR检测则显示氯丙嗪能引起Bcl-2、Bax、P53mRNA表达水平和Bax mRNA/Bcl-2 mRNA比值增高,除低剂量组的Bax、FSHRmRNA表达水平和Bax mRNA/Bcl-2 mRNA比值无明显改变外,其余各组均有统计学意义(P<0.01)。【结论】氯丙嗪可显著抑制大鼠卵巢颗粒细胞活力,诱导颗粒细胞凋亡。更多还原

【Abstract】 【OBJECTIVE】To investigate the relationship between female gonad toxicity of chlorpromazine on rat and the apoptosis of granulosa cells.Immature ovarian granulosa cell of Wistar rat was primary cultured.【METHOD】Cells were cultured and treated with diff-concentration chlorpromazine(0,0.1,1,10μM) for 24 h. After exposure to chlorpromazine 24h,cell viability was detected by MTT assay,apoptotic cells were identified microscopically by chromatin condensation and nuclear fragmentation with Hoechst 33258,mRNA expressions of Bax,Bcl-2 and P53 were determined by reverse transcriptase-polymerase chain reaction(RT-PCR).【RESULTS】Compared to control group,chlorpromazine could inhibit the proliferation of ovarian granulosa cell(P<0.01) in a concentration-dependent way.RT-PCR detection showed that chlorpromazine upregulated the mRNA expression of Bax,P53,FSHR and the mRNA ratio of Bax/Bcl-2.Compared with control group,the differences between relevant indicators was statistically significant(P<0.01) except Bax and Bax/Bcl-2 of low group.【CONCLUSION】Chlorpromazine may serve as an important endocrine disrupting medicine,mediating rat granulose cell apoptosis in vitro.The effect is bidirectional and concentration dependent.更多还原