【作者】 金桂华； 樊金娟； 刘长远； 赵奎华； 梁春浩； 王平；

【Author】 JIN Gui-hua~1,FAN Jin-juan~2,LIU Chang-yuan~1,ZHAO Kui-hua~1,LIANG Chun-hao~1,WANG Ping (1.Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China; 2.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China)

【机构】 辽宁省农业科学院植物保护研究所； 沈阳农业大学植物保护学院；

【摘要】 依据已克隆植物抗病基因的保守氨基酸结构域合成9条相应简并引物,通过PCR扩增从葡萄基因组DNA中分离了2条葡萄抗病基因同源序列GRA5、RGA16,通过对其编码的氨基酸序列分析表明二者同时含有P-环(GGVGKTT)、kinase-2(VLDD)、kinase-3(GSRII)及HD(即GLPLAL)保守域结构。经BLAST分析表明,分离的葡萄RGAs与已报道的植物的RGAs有较高的同源性。葡萄RGA的分离将为进一步从葡萄中分离功能性抗病基因打下基础,也为研究葡萄种质资源的起源与进化提供借鉴。更多还原

【Abstract】 According to the conserved amino acid domains(P- loop and GLPL) that exist in disease-resistance genes(R- genes),nine degenerated primers had been synthesized.2 NBS(nucleotide binding site) types of resistance gene analogues(GRA5、RGA16) from the grape were obtained by PCR (polymerase chain reaction) amplification.The amino acid sequences translated from GRA5、RGA16 both contain conserved domains,such as P-loop、kinase-2(VLDD)、kinase-3(GSRII) and GLPL. BLAST analysis showed that they have high homology with RGAs from other plants.These RGAs isolated from the grape used in present study would provide the base for the further cloning of disease-resistance genes in grape and provide a clue for the origin and evolution of grape- germ plasma.更多还原