【作者】 刘命华； 齐洪华； 吕睦廸； 李捷； 王克双； 程玉琴；

【Author】 Liu minghua~1,Qi honghua~2,L(u|¨) mudi~1,Li qie~1,Wang keshuang~1,Cheng yuqin~1 (1.Pomology/Lab of Stress Physiology and Molecular Biology for Tree Fruits,A key Lab of Beijing Municipality,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193; 2.Department of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)

【机构】 中国农业大学农学与生物技术学院果树系/北京市果树逆境生理与分子生物学实验室； 北京农学院植物科学技术学院；

【摘要】 克隆了82个葡萄卷叶伴随病毒3号(Grapevine leafroll-associated virus 3,GLRaV-3)中国分离物的CP基因全序列,并对我国葡萄园中的GLRaV-3天然群体(包括本文得到的82个和已报道的其他3个GLRaV-3中国分离物)进行了遗传多样性分析。结果表明,GLRaV-3中国分离物核苷酸和由此推导的氨基酸序列同源性分别为89.6%～100.0%和93.6%～100.0%,其平均核苷酸多样性度为0.0258。统计分析结果显示d_N/ds比值小于1,表明GLRaV-3 CP基因处于负向或纯化选择;且中国分离物间不存在重组可能性。以CP基因构建进化树分析表明,GLRaV-3中国分离物可分为4组,其中大多数分离物属于第一组,且聚类结果与样品产地和葡萄品种没有相关性,说明缺乏政府有效管控的葡萄苗木和繁殖材料的交换是该病毒传播的主要途径,且不同葡萄基因型可能缺乏对GLRaV-3的抗性基因,也无法选择某一特定的GLRaV-3变异体。这些结果对于建立更有效的GLRaV-3检测体系及采取合理的病毒防控措施具有较好的参考价值。更多还原

【Abstract】 CP genes of 82 GLRaV-3 isolates were cloned and the genetic variability of natural populations of Chinese isolates(82 obtained in this work and 3 from previous reports) was characterized.In pairwise comparisons,the CP sequences showed identities 89.6%to 100.0%and 93.4%to 100.0%at nucleotide and amino acid levels,respectively.The average nucleotide diversity for population of GLRaV-3 isolates in China was estimated as 0.0258,and no evidence for positive selection or recombination among Chinese isolates in this study was observed.A phylogenetic analysis suggested Chinese GLRaV-3 isolates were segregated into four distinct phylogenetic clades that did not reflect the vineyard origin of the isolates or an association with cultivar,indicating that uncontrolled exchange of infected plant material have been important in the dissemination of the pathogen,and most Vitis species might lack resistance to select for certain genetic variants.The results could be helpful to develop sound diagnostic systems and efficient and stable control measures.更多还原