【作者】 王钱东； 朱洁瑾； 张璐； 阮丽娜； 潘建春；

【Author】 WANG Qian-dong 1,2,ZHU Jie-jin 1,ZHANG Lu 1,RUAN Li-na 1,PAN Jian-chun 1 (1.Department of pharmachology,Wenzhou Medical College,Wenzhou 325035 2.Department of Anesthesiology,People’s Hospital of Ruian)

【机构】 温州医学院药学院； 瑞安市人民医院麻醉科；

【摘要】 目的研究低剂量漆黄素与阈下剂量胡椒碱急性给药的抗抑郁作用,初步探讨抗抑郁的作用机制。方法采用经典的行为绝望抑郁模型,即小鼠强迫游泳实验和悬尾游泳实验,观察低剂量漆黄素与阈下剂量胡椒碱急性给药对小鼠绝望模型不动时间的影响,同时观察两药联用对小鼠自主活动的影响。应用神经化学检测方法,观察漆黄素与阈下剂量胡椒碱急性给药对单胺氧化酶活性的影响。结果漆黄素(1.25mg·kg-1,2.5mg·kg-1,5mg·kg-1,p.o.)与阈下剂量胡椒碱(2.5mg·kg-1,i.p.)联合应用能显著减少小鼠强迫游泳和悬尾实验中的不动时间,且实验剂量范围内药物不影响小鼠的自主活动。进一步深入研究发现造成这种结果的原因是低剂量漆黄素与阈下剂量胡椒碱合用能剂量依赖性地抑制脑内MAO-A的活性。结论低剂量漆黄素与阈下剂量胡椒碱联合用药具有抗抑郁作用,其机制可能与抑制脑内单胺氧化酶活性,进而增加脑内胺类神经递质有关。更多还原

【Abstract】 Objective:The purpose of this study was to explore the antidepressant effects of low dose fisetin combined with piperine and its possible mechanism.Method:The animals behavioral despair models of depression,the tail suspension tests and forced swimming,was used to explore the antidepressant effects of low dose fisetin combined with piperine.In addition,the locomotor activity test was used to detect the change of locomotor activity.The monoamine oxidase activity in the mouse brain was also determined.Result:The results showed that fisetin (1.25mg·kg -1,2.5mg·kg -1 and 5mg·kg -1,p.o.) combined with piperine(2.5mg·kg -1,i.p.) significantly reduced the duration of immobility in both tail suspension and forced swimming tests.The effective doses did not alter locomotor activity.In addition,the combination administration dose dependently inhibited MAO-A activity.Conclusion:These findings suggest that low dose fisetin combined with piperine exerts antidepressant effect in animals behavioral despair tests and the underlying mechanism may involve the inhibition monoamine oxidase activity in the mouse brain.更多还原