【作者】 雷雪； 杨云； 刘富岗； 王俊敏；

【Author】 LEI Xue,LIU Fu-gang,YANG Yun,WANG Junmin (Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China)

【机构】 河南中医学院药学院；

【摘要】 目的优选纯化贯叶连翘提取物的大孔吸附树脂。方法以金丝桃素为指标,考察AB-8、S-8、CAD-45、D101、DA-201、DB-301、HPD-600、LSA-10、LSA-20、SP-825、XDA-6共12种不同型号的大孔吸附树脂对贯叶连翘提取物的纯化效果。结果所选出的LSA-10型大孔吸附树脂对金丝桃素的静态饱和吸附量可达1.9240mg·g-1,静态解析率达98.9%,动态比上样量为3.9083、比吸附量3.8540、比洗脱量2.8970。结论 LSA-10型大孔吸附树脂性能良好,可高效的纯化贯叶翘提取物。更多还原

【Abstract】 OBJECTIVE To select the optimum macroporous resin for the purification of Hypericum extracts.METRODS Taking hypericin as the index,and the purification effect of 12 types of macroporous resins,such as AB-8、S-8、CAD-45、D101、DA-201、DB-301、HPD-600、 LSA-10、LSA-20、SP-825、XDA-6、860021,were used to investigating the extracts of Hypericum perforatum.Results The selected LSA-10 macroporous resin was determined as follow:the staturated absorption capacity was 1.9240 mg·g -1,the desorption ratio was 98.9%;the dynamic sample content ratio was 3.9083,the absorption ratio was 3.8540,the elution content ratio was 2.8970.CONCLUTION LSA-10 macroporous resin had an obvious better efficiency,which is more effective for separation and purification of Hypericum extracts.更多还原