【作者】 周珊宇； 吴晓瑛； 刘涛； 谢传波； 王辉； 邱泉； 丁鹏； 陈维清；

【Author】 ZHOU Shan-yu,WU Xiao-ying,LIU Tao,XIE Chuan-bo,WANG Hui,QIU Quan,DING Peng,CHEN Wei-qing.Department of Biostatistics and Epidemiology,School of Public Health,Sun Yat-Sen University,74,Zhongshan Road 2,510080,Guangzhou,China.

【机构】 中山大学公共卫生学院医学统计与流行病学系；

【摘要】 目的探讨吸烟量与CHRNA7基因多态性对高血糖的联合作用。方法调查909名男性吸烟者的社会人口学信息及吸烟行为等信息,并采静脉血检测空腹血糖及CHRNA7上SNP位点rs2337980的多态性。在控制年龄、职业和糖尿病家族史后,应用多因素logistic回归模型,分析吸烟量和CHRNA7基因多态性对高血糖的联合作用。结果 909名男性吸烟者中,有166人(18.3%)为高血糖,743人(71.7%)血糖正常。在控制年龄、职业和糖尿病家族史等后,每日吸烟量>15支的男性比每日吸烟量为1～15支者患高血糖症的风险显著增高(OR=1.51,95%CI=1.06～2.14),携带变异基因型(CC/CT)的个体较携带rs2337980野生基因型(CC)患高血糖症的危险性显著增高(OR=1.74,95%CI=1.22～2.48)。在控制年龄、职业、糖尿病家族史和吸烟年数后,进一步根据CHRNA7上SNP位点rs2337980的多态性和每日吸烟量进行分层分析,以携带rs2337980野生基因型且每日吸烟量1～15支者为参照组,携带野生基因型且每日吸烟量>15支者、携带变异基因型且每日吸烟1～15支者和携带变异基因型且每日吸烟者量>15支者患高血糖症的危险性呈递增趋势,OR值分别为1.81(95%CI=1.07～3.07)、2.06(95%CI=1.26～3.38)和2.52(95%CI=1.52～4.17)。但未发现每日吸烟量和rs2337980多态性对高血糖症有交互作用。结论每日吸烟量与CHRNA7基因多态性对高血糖有联合作用。更多还原

【Abstract】 Objective To explore the joint effects of smoking quantity and α7 nicotinic acetylcholine receptor subunit gene(CHRNA7) on hyperglycemia.Methods A total of 909 male smokers were interviewed with a structured questionnaire about socio-demographic status and smoking behavior.Venous blood samples were collect to measure fasting serum glucose and a single nucleotide polymorphisms(SNPs) rs2337980 in CHRNA7 were measured with iMLDR method.A series of multivariate logistic regression models were performed to assess the join effects of smoking quantity and CHRNA7 gene polymorphisms on hyperglycemia with adjustment for age,occupation and family history of diabetes.Results There were 166(18.3%) hyperglycemia and 743(71.7%) normoglycemia individuals in 909 male smokers.Multivariate logistic regression analysis indicated that individuals smoking >15 cigarettes per day significantly increased the risk of hyperglycemia(OR=1.51,95%CI=1.06 ～ 2.14) than those smoking 1 ～ 15 cigarettes/day after adjusting the potential confounders.In addition,compared to those with wide type(CC) of rs2337980,individuals with variant type(CT/TT) of rs2337980 had an increased risk of hyperglycemia(OR=1.74,95%CI=1.22～2.48).A further stratified analysis by smoking quantity and rs2337980 genotypes indicated that compared with individuals smoking 1～15 cigarettes per day and carrying wild type of rs2337980,the increased risk of hyperglycemia was observed in ones smoking >15 cigarettes per day and carrying wild type(OR=1.81,95% CI=1.07～3.07),ones smoking 1～15 cigarettes per day and carrying variant type(OR=2.06,95%CI=1.26～3.38) and ones smoking >15 cigarettes per day and carrying variant type(OR=2.52,95%CI=1.52 ～ 4.17) after adjusting for the potential confounders.But,it was not found that smoking quantity and rs2337980 polymorphisms interactively affected on hyperglycemia.Conclusions These findings suggested that smoking quantity and CHRNA7 polymorphisms might exert the synergistic effects on hyperglycemia.更多还原