【作者】 赵丹萍； 陶彬； 陈立功； 李艳琴； 宋勤叶； 李潭清； 杨芳； 李红园； 袁洪兴；

【Author】 ZHAO Dan-ping1,TAO Bin1,CHEN Li-gong,LI Yan-qin,SONG Qin-ye2,LI Tan-qing,YANG Fang,LI Hong-yuan,YUAN Hong-xin(Experimental Station of Animal Pathogen Biology,Ministry of Agriculture /Agricultural University of Hebei,Baoding Hebei 071000,China)

【机构】 河北农业大学农业部动物疫病病原生物学华北区观测实验站；

【摘要】 (目的)为了探索猪圆环病毒2型(PCV2)感染对猪瘟(CSF)弱毒疫苗接种猪免疫应答的影响。(方法)将20头28d断奶仔猪随机分为V-I、I-V、V和C四组,5头/组。V-I组在接种CSF弱毒疫苗后2d感染PCV2;I-V组在感染PCV2后2d接种CSF弱毒疫苗;V组只接种CSF弱毒疫苗;C组为空白对照组。共免疫2次,间隔21d。免疫后定期检测血清CSFV特异性抗体水平、外周血淋巴细胞(PBLC)增殖活性和PBLC中IFN-γ、IL-2、IL-4和IL-10mRNA的表达水平。(结果)结果显示:在CSF弱毒疫苗免疫前或免疫后感染PCV2,均会导致CSFV抗体水平低下,血清抗体阳转率下降,PBLC增殖活性降低。初次免疫后,V-I与I-V组的PBLC内IFN-?、IL-2、IL-4和IL-10mRNA的表达量严重不足,其中V-I组的表达量最低。加强免疫后V-I与I-V组的PBLC内IFN-γ与IL-10的表达失衡,IL-2和IL-4的表达缺乏,其中I-V组的细胞因子表达失衡和缺乏更严重。(结论)上述研究表明,CSF弱毒疫苗免疫前或免疫后感染PCV2均会影响机体的体液和细胞免疫应答水平,导致PBLC内细胞因子的表达严重抑制和紊乱。更多还原

【Abstract】 To study the influence of porcine circovirus type 2(PCV2)infection on immune response of pig to classical swine fever(CSF)vaccine,twenty 28-day-old healthy weaning piglets were randomly divided into four groups of five(Group V-I,I-V,V and C).The piglets in Group V-I were intramuscularly injected with CSF vaccine two days before being inoculated with PCV2,Group I-V infected by PCV2 two days before being injected with CSF vaccine,Group V only vaccinated by CSF vaccine and Group C as the blank control without inoculating anything.Piglets were vaccinated twice at an interval of twenty-one days and blood samples were collected periodically so as to detect CSFV-specific serum antibody,lymphocyte proliferation activity and mRNA expression levels of IFN-γ,IL-2 and IL-4 in peripheral blood lymphocyte(PBLC).PCV2 infection,either before or after vaccination,decreased CSFV-specific serum antibody production,seroconversion and lymphocyte proliferation activity.After the primary vaccination,expression of IFN-γ,IL-2,IL-4 and IL-10 mRNA decreased seriously in PBLC from piglets in Group V-I and Group I-V,especially that of Group V-I.Following the secondary vaccination,expressions of IFN-γ and IL-10 mRNA in PBLC from Group V-I and Group I-V appeared seriously unbalanced and those of IL-2 as well as IL-4 mRNA were deficient,especially those of Group I-V.In conclusion,PCV2 infection before or after vaccination with CSF vaccine affected porcine humoral and cellular immunity and led to expression suppression and unbalance of cytokines in PBLC.更多还原