【作者】 张琳； 胡北侠； 张秀美；

【Author】 ZHANG Lin,HU Bei-xia,JIANG Yi-nan,XU Chuan-tian,YAN Shi-gan,YANG Shao-hua,HAO Ming-fei,ZHANG Xiu-mei(1.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Science,Jinan 250100,China;2.The Base of Police Dog,Chongqing Public Security Bureau,Chongqing 400020;3.College of Animal Science,Shandong Agriculture University,Taian,Shandong 271018,China)

【机构】 山东农业科学院畜牧兽医研究所；

【摘要】 从规模化养鸭场分离到4株鸭坦布苏病毒,成功克隆了包膜蛋白(E蛋白)基因片段,并对其进行了序列测定、分析和表达。结果表明,E基因全长1503bp,编码501个氨基酸,序列高度保守。进化分析显示,鸭坦布苏病毒属蚊媒黄病毒类恩塔亚病毒群,与其他坦布苏病毒及近期发现的BYD病毒关系最近。采用原核表达系统对E基因进行了表达,蛋白大小54.3kDa,主要以包涵体形式存在。随后,对E蛋白外功能区序列和3D结构进行了分析,其在已解析结构的黄病毒中与WNVE结构最为相似。研究结果为该病的防控和基因工程疫苗的研制奠定了基础。更多还原

【Abstract】 Envelop proteins(E protein)genes of four duck TMUV isolated from broilers were cloned,sequenced and expressed.E gene was comprised of 1503 nucleotides,encoding 501amino acid.Phylogenetic analysis shows that duck TMUV belongs to mosquito-born flavivirus,NTAV group,similar with other Tembusu and BYD virus.The molecular weight of E protein was 54.3kDa expressed in pET21a/BL21(DE3)system.The sequence and three-dimensional structure of ectodomain of duck TMUV were most similar with WNV E protein.The result was useful for the control and the development of molecular vaccine of duck TMUV.更多还原