【作者】 黄贻涛； 张子平； 王国栋； 邹志华； 王淑红； 王艺磊；

【Author】 HUANG Yitao 1 , ZHANG Ziping 2 , WANG Guodong 1 , ZOU Zhihua 1 , WANG Shuhong 1 , WANG Yilei 1 (1.Fisheries College, Jimei University, Xiamen, China, 361021; 2.Department of Biological Science, Seton Hall University, New Jersey, 07079, USA)

【机构】 集美大学水产学院； 西东大学生物系；

【摘要】 众多的研究表明,热休克蛋白(Heat shock protein,HSP)的含量与生物的耐热性呈正相关,且能够提高生物的应激能力和在逆境中的存活率。在热应激反应中,有一类结构较为特殊的蛋白家族---热休克因子结合蛋白(Heat shock factor binding protein,HSBP),参与了热激蛋白基因表达的调控过程,对热激蛋白的表达起负调控作用。本文首次克隆了杂色鲍HSBP基因cDNA全序列,其全长731bp,开放阅读框为225bp,编码75个氨基酸。实时定量PCR结果显示,HSBP在杂色鲍各个组织中都有表达,其中血淋巴和鳃中表达量最高。以31℃高温对杂色鲍进行应激处理,我们发现:随着温度的升高,鳃中该基因表达量下降,31℃条件下4h时,该基因表达量达到最低。随后其表达量迅速上升,并于24h达到最高。96h和192h检测该基因表达量又逐渐下降。各时相中,高温应激组的HSBP表达量都要低于对照组(25℃)。结果表明HSBP在杂色鲍热激调控中起到一定负调控作用。更多还原

【Abstract】 Numerous studies show that the content of HSPs and biological heat resistance were positively correlated. HSPs increase resistance to stress and survival rate of organisms in adversity. Under thermal stress, a special protein family-Heat shock factor-binding protein (HSBP), participates in the gene expression regulation of heat shock protein, and has negative impacts on the expression of heat shock protein. In this study, Hd-HSBP gene was cloned from Haliotis diversicolor. The full-length cDNA sequence of Hd-HSBP is 731bp with a 225 open reading frame that encoding a protein of 75aa. The tissue distribution and its expression of Hd-HSBP under thermal stress were measured by quantitative real-time PCR. The Hd-HSBP was detected in all examined tissues, with the highest level in hemocyte and gill. Under the thermal stress of 31℃, the Hd-HSBP was down-regulated in gill as temperature-rise period (25 ℃ to 31℃). When up to 31℃, the expression level of Hd-HSBP achieved the lowest at 4 hours. Soon afterwards, the expression level of Hd-HSBP rose rapidly at 24h, and then decreased at 96h and 192h. Meanwhile, the expression level of Hd-HSBP at high temperature stress was lower than the control group at temperature of 25℃ in all time courses. This result may indicate that the hd-HSBP plays a negative role in thermal stress in H. diversicolor.更多还原