【作者】 王晓伟； 高洁； 邹志华； 贾锡伟； 林鹏； 张子平； 王艺磊；

【Author】 WANG Xiao-Wei 1 , GAO Jie 1 , ZOU Zhi-Hua 1 , JIA Xi-Wei 1 , LIN Peng 1 , ZHANG ,Zi-Ping 2 ,WANG Yi-Lei 1* (1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Department of Biological Science, Seton Hall University, New Jersey, 07079, USA)

【机构】 集美大学水产学院； 西东大学生物系；

【摘要】 拟穴青蟹(Scylla paramamosain)是我国重要的经济养殖蟹类,其生殖调控机理的研究受到越来越多的关注。ERK途径是调控性腺发育的重要信号通路。本研究利用SMARTRACE技术克隆得到ERK信号通路相关的G蛋白β1(Gnb1)和酪氨酸3-加单氧酶/色氨酸5-加单氧酶激活蛋白(14-3-3蛋白)基因。14-3-3蛋白能与磷酸化的蛋白结合,参与发育、分化、生殖等过程,其cDNA全长1092bp,编码248个氨基酸。Gnb1是G蛋白重要组分,在细胞外信号向细胞内信号转导的过程中起关键作用。其cDNA全长1487bp,编码340个氨基酸。实时定量PCR结果显示Gnb1和14-3-3在各组织器官均有表达,但在卵巢中的表达量显著高于其他组织,推测它们在卵巢中发挥重要作用。更多还原

【Abstract】 Green mud crab, Scylla paramamosain, is an important commercial species in aquaculture. Therefore, more and more researches focus on its molecular mechanism of reproduction. ERK pathway is essential for the regulation of gonad-development.The full length cDNAs of tyrosine-3-monooxygenase/tryp tophane-5-monooxygenase activator protein (14-3-3 protein) and G Protein Beta 1 (Gnb1), which belong to ERK pathway, were obtained by SMART RACE in this study. The 14-3-3 was approved to be bonded to phosphorylated protein and involved in the process of development, differentiation and reproduction etc. The full length cDNA of 14-3-3 gene is 1092bp that encoding a polypeptide of 248 amino acids. Gnb1 is an essential component of G protein, and plays a key role in the procession which translate extracellular signal to intracellular. The full length of Gnb1 is 1487bp that encoding a polypeptide of 340 amino acids. The results of real time quantitative PCR indicated that the expression level of both genes in ovary was significantly higher than other examined tissues. Consequently, we suggest that Gnb1 and 14-3-3 may play a key role in ovary.更多还原