【作者】 苗贵东； 徐营； 汪娣； 陈松林；

【Author】 MIAO Gui-Dong~(1,2),XU Ying~2,WANG Di~2,CHEN Song-Lin~2 (1 Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences Wuxi,214081,China 2 Key Lab for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071,China)

【机构】 中国水产科学研究院淡水渔业研究中心； 中国水产科学研究院黄海水产研究所；

【摘要】 用9个微卫星标记组合建立了2个微卫星多重PCR体系,对半滑舌鳎16个人工选育家系进行了系谱认证、亲子鉴定和遗传多样性研究。2个多重PCR体系中9个微卫星位点共检测到57个等位基因,每个位点等位基因数为3～11个。根据已知亲本及子代基因型,成功推断出了2个缺失母本的基因型。在双亲未知的情况下2个多重PCR的9个微卫星位点累积排除概率是95.66%,已知1个亲本时累积排除率为99.65%,亲子鉴定准确率为90%。利用UPGMA法对16个家系的160个体进行了聚类分析,同一家系93.13%的个体聚类分析结果与系谱来源一致。2个多重PCR体系的建立为半滑舌鳎不同家系混养后的分子辅助家系管理、亲子鉴定、系谱分析和分子辅助亲本选择提供了更高效的技术手段。更多还原

【Abstract】 We developed two microsatellite multiplex PCR systems using 9 microsatellite loci for Half-smooth tongue sole(Cynoghssus semilaevis),one important aquaculture species in China.The multiplex PCR microsatellite assay was used to estimate the kinship and the genetic diversity of sixteen Cynoghssus semilaevis families.The result showed the number of alleles ranged from 3 to 11,57 alleles were obtained in 9 loci.Based on the genotypes of either known parent or none of them and offspring,all the absent parental genotypes of the seven families were deduced.According to the unique alleles among the families,each family could be distinguished from the others.Cervus2.0 analysis showed that the combined exclusion probability was 95.66%if parents were both unknown and the combined exclusion probability was 99.65%when only one of the parental genotype was known.The UPGAM dendrogram of the 160 individuals indicated that 93.13%of the individuals from the same family dusted together.The results showed that microsatellite multiplex PCR is an useful tool for parentage determination,,genealogical identification and molecular assisted parent selection of Cynoghssus semilaevis.更多还原