【作者】 麻艳群； 黄凯； 卢克焕； 韦友传； 周昕期；

【Author】 MA Yan-qun~(1,2),VHUANG Kai~1,LU Ke-huan~(1,2),WEI You-chuan~1,ZHOU Xin-qi~1 (1.College of Animal Science and Technology,Guangxi University,Nanning 530005,China; 2.Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization,Guangxi University,Nanning 53005,China)

【机构】 广西大学动物科技学院； 广西亚热带生物资源保护利用重点实验室；

【摘要】 本研究旨在探讨Cu²⁺浓度梯度、胁迫时间对禾花鲤（Procypris mterus）血液和肝脏的过氧化氢酶（Catalase,CAT）活性的影响,为禾花鲤的重金属胁迫研究及水污染生物监测提供理论参考依据,进一步指导禾花鲤的健康养殖。采用可见光法测定了不同Cu²⁺浓度（0mg/L、0.067mg/L、0.084mg/L、0.101mg/L、0.118mg/L、0.134mg/L）和胁迫时间（0d、1d、5d、11d）下禾花鲤CAT活性的变化。结果显示,在试验的各个持续时间段（1d、5d、11d）,低Cu²⁺浓度处理组（0.067mg/L）血液及肝脏的CAT活性高于其它各处理组,且活性随着胁迫时间的延长而增加。而中、高Cu²⁺浓度处理组（0.084mg/L、0.10lmg/L、0.118mg/L、0.134mg/L）血液和肝脏中的CAT活性则随着Cu²⁺浓度的升高和胁迫时间的延长被显著抑制（P<0.05）,活性变化在5d后趋于稳定。试验表明鱼体过氧化氢酶的活性受水体中Cu²⁺浓度的影响,低Cu²⁺浓度能诱导鱼体血液和肝脏中的CAT活性增加,但随胁迫的加剧,CAT活性受到抑制。更多还原

【Abstract】 The aim of this experiment was to examine the effects of copper stress on the catalase（CAT） activity in blood and liver tissue of Carp（Procypris mterus）.Carps were treated with 0mg/L、0.067mg/L、0.084mg /L、0.101mg/ L、0.118mg/L、0.134mg/L of Cu²⁺ for periods of 0 to 11d under visible light condition.The results showed that highest CAT activities in blood and liver tissue were observed in the lower Cu²⁺ concentrations treatment（0.067mg/L） and CAT activities of this treatment were increased with exposure time.However,CAT activities in blood and liver tissue in other treatments(0.084mg/L、0.101 mg/ L、0.118mg/L、0.134mg/L Cu²⁺ concentrations) were decreased with Cu²⁺ concentrations and exposure time （P<0.05） and tended to be stable after 5 days.The experiment suggests that CAT activity can be affected by Cu²⁺ concentrations in water,and a low dose stimulation followed by a high dose inhibition.更多还原