【作者】 李莉； 程龙献； 廖玉华；

【Author】 LI Li,CHENG Long-xian,LIAO Yu-hua(Department of Cardiology,Union Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430022)

【机构】 华中科技大学同济医学院附属协和医院心内科； 武汉大学人民医院心内科；

【摘要】 目的:研究牵张心室时牵张激活通道(SACs)对心肌细胞表达原癌基因c-fos的影响。方法:离体灌流大鼠心脏并膨胀左心室30 m in,停止灌流剪下左心室,抽提组织总RNA行半定量RT-PCR,探讨牵张左室对心室细胞表达c-fos的影响及SACs在其中的可能作用。结果:RT-PCR结果示,在同样牵张力度及持续时间膨胀心室的条件下,牵张加链霉素组c-fos表达水平明显低于无链霉素组。结论:SACs通道阻断剂-链霉素对膨胀左室致c-fos表达有明显抑制作用,间接说明心室膨胀经SACs激活胞内信号诱导c-fos表达;同时链霉素并不能完全抑制c-fos表达,提示膨胀心室致c-fos表达进而引起心室肥厚尚有其他传导途径,仍需进一步研究。更多还原

【Abstract】 AIM: To investigate the effects of stretch-activated channels(SACs) on ventricular c-fos expression.METHODS: Isolated normal adult rat hearts were perfused and streched by Langendorff method.The left ventricles were cut off after 30 min reperfusion.The total RNA was extracted for RT-PCR to determine c-fos expression.RESULTS: The level of c-fos expression in streptomycin group was significantly lower than that of the group without streptomycin.CONCLUSION: Streptomycin,a blocker of SACs,can partially inhibit left ventricular dilation-induced c-fos expression,impling that SACs may be one of the pathways to induce c-fos expression and ventricular hypertrophy.更多还原