GmZFP和GmTIP协同调控大豆耐非生物胁迫的分子机理研究

【作者】 张大勇； 易金鑫； 许玲； 徐照龙； 何晓兰； 黄益洪； 刘晓庆； 马鸿翔；

【机构】 江苏省农业科学院农业生物技术研究所江苏省农业生物学重点实验室； 南京农业大学农学院；

【摘要】 锌指蛋白和水通道蛋白基因是植物应答逆境胁迫的重要基因,我们从大豆中克隆了一个带有锌指结构蛋白的基因GmZFP和一个水通道蛋白基因GmTIP,发现它们受非生物胁迫诱导,对GmZFP和GmTIP蛋白的研究表明它们都主要定位于细胞膜上,而且二者是互作的;在逆境条件下,GmZFP可能充当转录因子直接结合到GmTIP启动子部位抑制其表达来共同调节植物对外界胁迫的耐性;抑制GmZFP和过表达GmTIP的组合植株都能有效地提高对干旱与盐胁迫的耐性。本文将进一步研究GmZFP和GmTIP协同调控大豆耐非生物胁迫的分子机理研究,为通过转基因技术创造一些具有理论研究和实际应用价值的大豆转基因新种质提供宝贵的基因资源,同时对进一步深入探究胁迫信号转导的网络体系具有重要的理论意义。更多还原

【Abstract】 ZFP(Zinc finger protein)genes and Aquaporins genes play important roles in plant response to reverse stress,in this study,we cloned a Glycine max GmZFP and a GmTIP(tonoplast protein),both of them could be induced by abiotic stresses.Subcellular localization and BIFC (bimolecular fluorescence)experiments mainly located at the cell membrane and interacted with each other.However,under the stress condition,GmZFP maybe act as a transcript factor and directly bind to the promoter of GmTIP to cooperatively regulate the tolerance of plant to stresses by suppressing its expression;the composite plants of silencing GmZFP and over-expression GmTIP can effectively increase the tolerance to drought and salinity stresses.This project will further insight into the molecular mechanism of cooperative regulation of GmZFP and GmTIP,which will provide valuable gene resources for theoretical and applied researches of transgenic soybean novel germplasms via transformation technology,and will provide a new theoretical basis for stress signal transduction web system.更多还原