【作者】 张红漫； 李志惠； 吴树才； 刘朋冲； 赵杰； 李毅； 崔丹； 冯秀莉；

【Author】 Zhang hongman,Li zhihui,Wu shucai,Liu pengchong,zhao jie,Li yi,Cui dan,Feng xiuli Heibei Chest Hospital,Shijiazhuang050041,China

【机构】 河北省胸科医院；

【摘要】 目的探讨荧光定量PCR技术检测胸腔积液结核分枝杆菌DNA对结核性胸膜炎的诊断价值。方法对112例肺结核并结核性胸膜炎患者、149例结核性胸膜炎和49例非结核性胸腔积液标本行荧光定量PCR检测结核分枝杆菌DNA。结果 112例肺结核并结核性胸膜炎患者胸腔积液荧光定量PCR检测阳性43例(38.39%),149例结核性胸膜炎患者胸腔积液荧光定量PCR检测阳性54例(36.24%),49例非结核性胸腔积液患者荧光定量PCR检测阳性0例(0%)。3组阳性率比较,差异有统计学意义(P<0.05)。结论胸腔积液荧光定量PCR检测对结核性胸膜炎诊断有重要价值。更多还原

【Abstract】 Objective:To assess the diagnostic value of the quantitative measurement of fluorescence quantitative PCR in diagnosis of tuberculous pleurisy。Method:Detecting the TB-DNA in 112 cases with both tuberculosis and tuberculous pleurisy,149 cases with tuberculous pleurisy and 49 cases with non tuberculous pleural effusion by fluorescence quantitative PCR.Results:Positive result of the fluorescence quantitative PCR was found in 43 out of 112 cases with both tuberculosis and tuberculous pleurisy,40 out of 149 cases with tuberculous pleurisy and non of 49 cases with non tuberculous pleural effusion.There was statistical significance among three groops。(P<0.05);Conclusion:The value of fluorescence quantitative PCR from pleural effusions in diagnosis of tuberculous pleurisy。更多还原