【作者】 马聪；

【Author】 MA Cong, et al. Centerfor Disease Control and Prevention of Guangdong Province,Guangzhou 510300,China

【机构】 广东省疾病预防控制中心；

【摘要】 目的研究广东地区多宗副溶血性弧菌暴发分离的优势血清型(03:K6、01:Kut、04:K8)株的毒素基因携带情况、"大流行群"株分布特征以及不同血清型株之间的相关性。方法对2008-2010年23起副溶血性弧菌暴发患者临床样本和当餐食品分离的62株优势Iln清型进行tdh、trh基因检测及"大流行群"鉴定(GS-PCR和o∥8),并利用Not I酶切的脉冲场凝胶电泳(PFGE)分型方法分析了其中44株的谱型相似性。结果 96.8%(60/62)的分离菌株毒素基因检测结果为tdh+,trh-;3株食品分离株携带tdh基因。分别有97.2%(35/36)03:K6型、5.88%(1/17)04:K8型、66.7%(8/9)的01:Kut型菌株鉴定为"大流行群"(GS-PCR+和/或0rf8+)。PFGE分型结果显示,44株分为3个聚类,属于"大流行群"聚类的28株之间的相似性为80.5%,与另外两个非"大流行群"聚类的相似性仅为59.5%;并发现部分"大流行群"03:K6型分离株与Ol:Kut、04:K8呈现一致的PFGE谱型。结论 2008-2010年广东地区副溶血性弧菌暴发分离的优势血清型株大多携带tdh毒力基因;"大流行群"株以03:K6和01:Kut型占优;在同一宗暴发中,归属"大流行群"的不同血清型菌株存在高度相似性。更多还原

【Abstract】 Objective To study the toxin genes and pandemic group distribution as well as the genetic correlation between tIle major serotypes(03:K6,01:Kut,04:K8)of Vibrio parahaemolyticus(VP) isolated from the outbreaks of Guangdong province.Methods The tdh and trh genes.GS-PCR and orf8 gene were detected on the 62 isolates sourced from patient and seafood occurred in the 23 outbreaks during 2008-2010.44 isolates of which were analyzed on PFGE digested by Not I enzyme.Results Toxin genes distributions suggested tIlat 96.8 %(60/62)isolates were tdh+,trh-.Three tdh+ isolates sourced from seafood were found.Pandemic group distribution suggested that 97.2%(35/36)03:K6,5.88%(1/17)04:K8,66.7%(8/9)01:Kut serotype was GS-PCR+ and/or orf+,respectively.PFGE analysis suggested that 44 isolates were separated into 3 clusters of which the similarity of PFGE profile was 80.5%in the pandemic group cluster constructed by 28 isolates,the similarity between pandemic group and non-pandemic group was 59.5%.Pandemic group of 03:K6,01:Kut as well as 04:K8 isolated on some outbreaks were processing the sarfle PFGE profiles.Conclusion The characteristic of toxin genes of major serotypes VP isolated in the outbreaks of Guangdong province form 2008-2010 was tdh-present and trh-absent.Within the pandemic Group,03:K6 and 01:Kut were the major serotypes.In single outbreak,isolates belongs to pandemic group but with different serotype seem to be close correlations.更多还原