【作者】 王重建； 杨年红； 许明佳； 武颂文； 杨雪锋； 刘烈刚； 孙秀发；

【Author】 Wang Chong-jian,Yang Nian-hong,Xu Ming-jia,Wu Song-wen,et al Department of Nutrition and Food Hygiene,School of Public Health,Tongji Medical College, Huazhong University of Science & Technology,Wuhan 430030,China

【机构】 华中科技大学同济医学院公共卫生学院营养与食品卫生学系；

【摘要】 目的研究高脂饮食诱导肥胖(DIO)及肥胖抵抗(DIO-R)大鼠血浆PYY3-36含量及回结肠PYYmRNA的表达情况,探讨PYY3-36与高脂饮食诱导肥胖抵抗的关系。方法 36只雌性SD大鼠,按体重随机分为高脂实验组(n=27)和基础对照组(n=9),分别给予高脂饲料和基础饲料喂养13周。据13周末体重将高脂饲料组再分为饮食诱导肥胖和饮食诱导肥胖抵抗组,比较各组大鼠能量摄入水平、血浆PYY3-36含量及回结肠PYY mRNA表达水平的差异。结果 DIO-R大鼠热能摄入量显著低于DIO大鼠(P<0.01),与对照组大鼠相比无显著性差异(P>0.05);DIO-R大鼠血浆PYY3-36含量及回结肠PYY mRNA表达水平显著高于DIO)和对照组大鼠(P<0.01);DIO与对照组相比,除回肠PYY mRNA表达水平升高外,其余指标均无显著性差异(P>0.05)。结论高脂饮食条件下,SD大鼠表现为明显的肥胖易感性差异,这种差异可能与PYY3-36的食欲调节密切相关,血浆PYY3-36含量和回结肠PYY mRNA表达水平的升高可能是导致DIO-R大鼠热能摄入下降的内在机制之。更多还原

【Abstract】 Objective To study the plasma concentration of peptide YY3- 36(PYY3 36) and the expression levels of PYY mRNA of ileum and colon in dietary induced obesity(DIO) and dietary induced obesity resistant(DIOR) rats and the relation between PYY3-36 and DIOR rats.Methods Thirty six female SD rats were randomly divided into high-fat diet group(n= 27) and chow fed control group(n= 9),after 13 weeks of either a high fat diet or chew fed diet,the liiglr fat diet group was subdivided into DIO and DIO-R group according to the final body weight.Weight gain,caloric intake,the concentration of PYY3 36 and the expression levels of PYY mRNA were measured and compared.Results The total caloric intake of DIO-R rats was lower than DIO rats(P<0.01),while no significant difference was found between DIO R and control rats(P>0.05).The concentration of PYY3-36 and the expressions of PYY mRNA of ileum and colon in DIO-R rats were higher significantly than that of the DIO and control rats(P<0.01),wliile no significant difference was found between DIO and control group(P>0.05),except that PYY mRNA of ileum was advanced in DIO group(P<0.01).Oondusion On the High fat diet,SD rats showed different susceptibility to obese and energy intake,increased levels of PYY3- 36 and PYY mRNA might be related to dietary induced obesity resistant.更多还原