【作者】 王福俤； 赵法伋； 郭俊生； 景乃禾；

【Author】 Wang Fudi,Zhao Faji,Guo Junsheng,Jing Nai he Department of Public Health,Second Military Medical U niversity,Shanghai 200433,China

【机构】 第二军医大学军队卫生学教研室； 中国科学院上海生物化学研究所；

【摘要】 为探讨发育期锌缺乏降低脑组织微管聚合作用的可能机理,作者观察了孕期及哺乳期锌缺乏母鼠其仔代成年时的学习能力以及脑组织α微管蛋白(α-Tub)、β-微管蛋白(β-Tub)和微管相关蛋白2(M AP2)表达水平。给处于孕期及哺乳期ICR母鼠喂饲含不同锌水平的实验饲料,饲料锌水平分别为1、5、30及100mg/kg。在穿梭箱内检测实验仔鼠成年(70日龄)时的学习能力,然后采用Western blot技术检测其脑组织中α-Tub、β-Tub及MAP2的表达情况。行为学检测发现缺锌组(1和5mg/kg)达到学会标准所需次数明显多于非缺锌组(30及100mg/kg),表明缺锌小鼠学习能力受损;比较各实验组α-Tub、β-Tub及MAP2表达量发现,缺锌组α-T ub、β-Tub及MAP2杂交信号明显弱于非缺锌组。蛋白表达量顺序均为1 mg/kg组<5mg/kg组<30mg/kg组<100mg/kg组,表明α-Tub、β-T ub及M AP2的表达水平与膳食锌呈正依赖关系。α-Tub、β-Tub及MAP2表达量降低可能是脑组织微管聚合作用下降的重要机制所在,并且与锌缺乏引发的脑功能损伤密切相关。更多还原

【Abstract】 To probe into the mechanism of zinc deficiency on microtubule polymerization impairment,the learning ability and the levels ofα-tubulin,β-tubulin and micro tubule-associated protein 2 expression in the brain of zinc deficient offsprings,maternal ICR mice were fed with experiment diets containing different levels of zinc(l,5,30 and lOOmg/kg) during pregnancy and lactation respectively.On the postnatal day 70 of offsprings,the learning ability and the expression ofα-tubulin,β-tubuhn and m icrotubule-associat ed protein 2 in the brain were examined by shuttle box and Western blot assays respectively.The results showed that the number of trials needed to reach the learning criterion for zinc deficient groups(1 and 5mg/ kg) was much higher than that for non zinc deficient groups(30 and lOOmg/kg).The levels ofα-tubulin,β-tubulin and microtubule -associated protein 2 expression in the brain of zinc deficient offsprings(1 and 5mg/kg) were lower than those in the brain of offsprings whose dams fed with zinc adequatedly supplied diet(30mg/kg) and zinc supplem ented supplied diet(100mg/kg) respectively-These result s suggested that the inhibition of Ortubulin,β-tubulin and microtubule-associat ed protein 2 expression might be the most important mechanism of microtubule polymerization decline resulting from zinc deficiency,which had close relationship with brain function impairment.更多还原