【作者】 涂国全； 刘姝； 黎循航；

【Author】 TU Guoquan LIU Shu LI Xunhang (Department of Bioengineering,Jiangxi Agricultural University,Nanchang 330045)

【机构】 江西农业大学生物工程系；

【摘要】 <正>通过梅岭霉素(Meilingmycin)产生菌南昌链霉菌NS-41-80菌株孢子对6种抗生素敏感性测定,采用诱变剂EMS 4种不同诱变剂量对菌株孢子进行诱变处理,诱变处理的孢子涂布在含致死浓度链霉素的高氏平板上。从抗药性突变标志菌株中进一步筛选梅岭霉素高产菌株。在1500多个抗药性突变株中通过初筛获得了比诱变出发菌株的产素能力提高50%以上菌株。通过诱变剂量分更多还原

【Abstract】 After testing the resistance of 6 antibiotics to the strain NS-41-80 which is the Meilingmycin producer,Streptomyces nanchangensis,about 1500 spore resistant chemicals mutants which resist to Sterptomyein were screened after the spores regenerated on the lethal media when they were treated with 4 different dosage of super-mutengent EMS.We have abtained spore mutants whose yields are 50 percent higher than CK’s among all mutants in the pre-screening experments.It was showed that the resistancechemical mutation of the strains had closely relation with yield mutation and the EMS dosage of the yield mutation was higher than the resistance-chemical mutation’s through the statistical analysis between the dosage of mutation and the resistance-chemical mutational frequency,the variate capacity of the mutants’ yield.The lethal frequency of the spores was 99.43%and the resistance-chemical mutational frequency was 0.0440%,and a model of the resistance-chemical mutational labelling rational selection of the strain producing Meilingmycin was established.It was a useful experiment of the study on the screening of the high-yield Streptomyces nanchangensis and it would help to the study on the screening of the other Streptomyces producing antibiotics.更多还原