【作者】 戴海英； 邢新； 薛春雨； 张培培； 梅香； 杨硕成； 李鸣；

【Author】 Dai haiying,XUE Chun-yu,XING Xin,Zhang Peipei, Meixiang,Yang Shuocheng,Li Ming Department of Plastic Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China

【机构】 第二军医大学附属长海医院整形外科；

【摘要】 目的检测钙调蛋白(calmodulin,CaM)在人自体移植中厚皮片中的表达,初步认识钙信号转导在自体移植皮片过度色素沉着发生机制中的作用。方法利用免疫组织化学方法,检测钙调蛋白在受区移植皮片、受区周围与供区周围正常皮肤中的表达并进行统计学分析。结果钙调蛋白的表达定位于表皮基底部黑素细胞、角朊细胞胞浆,在大部分自体中厚移植皮片中呈强阳性表达,与自体正常对照皮肤中的表达差异有显著性意义(P<0.05);钙调蛋白在受区和供区周围正常对照皮肤中的表达差异无显著性意义。结论钙调蛋白在自体中厚移植皮片中的表达较在自体正常对照皮肤中显著提高,推测钙信号转导在自体移植皮片色素过度沉着中起重要调控作用。更多还原

【Abstract】 Objective To investigate the expression of calmodulin in human split-thickness skin autografts and to recognize the role of calcium signal transduction in hyperpigmented process of the skin grafts. Methods Immunohistoehemieal technique was adopted to detect the expression of calmodulin in the split-thickness grafted skin,the normal skin around the recipient area and normal skin separately and statistics was used to analyze the data.Results The expression of calmodulin was located in endochylema and cytolemma of melanocytes and keratinocytes in the basilar part of epidermis.The expression of calmodulin in most of the split -thickness grafted skin was significantly higher than the control skin,The positive integral optical density of calmodulin in the split-thickness skin grafts(0.49±0.08) was significantly higher than that in the normal skin around the recipient site(0.23±0.08) and in the normal skin around the donor site(0.18±0.06);showing statistically significant difference(P<0.05).The expression of calmodulin in the normal skin of the donor area was no statistic remarkably differences compared to the normal skin around the recipient area.Conclusion The results indicate that the expression of calmodulin may markedly increase in the split-thickness grafted skin and correlate with its pigmentation affer the skin graft.Calcium signal transduction may play an important role in hyperpigmented process of the skin graft.更多还原