【作者】 曹文富； 陈益山； 焦颖华；

【Author】 CAO Wen-fu,CHEN Yi-shan,JIAO Ying-hua (Department of Integrated Traditional Chinese and Western Medicine,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

【机构】 重庆医科大学附属第一医院中西医结合科；

【摘要】 目的:观察解聚复肾宁对糖尿病肾病(DN)大鼠肾脏蛋白激酶C(PKC)、血管内皮生长因子(VEGF)和血小板衍生生长因子-B(PDGF-B)的影响及对肾脏的保护作用。方法:建立链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠模型,将成模糖尿病大鼠随机分成4组:模型组、解聚复肾宁组、厄贝沙坦组、厄贝沙坦+解聚复肾宁组,同时取正常大鼠设对照组。各组大鼠采用相应的干预措施处理12周。常规方法检测各组大鼠肾脏指数、血糖、尿素氮(BUN)、血肌酐(Scr)、尿白蛋白排泄率(UAER),免疫组化法检测肾PKC和VEGF表达,Western blotting检测肾组织PDGF-B的表达,透射电镜观察肾脏超微结构。结果:模型组大鼠肾皮质PKC、V EGF和PDGF-B表达显著升高,肾脏超微结构改变明显,血糖、UA ER、BUN、Scr、肾脏指数显著增高;解聚复肾宁组、厄贝沙坦组和厄贝沙坦+解聚复肾宁组上述指标显著低于模型组(P<0.05),肾脏超微结构改变明显改善;厄贝沙坦+解聚复肾宁组各项指标改善明显优于模型组、解聚复肾宁组和厄贝沙坦组(P<0.05),但未达到对照组水平。结论:解聚复肾宁对DN大鼠肾脏形态和功能有明显保护作用,其机制可能与减少肾组织中PKC、VEGF和PDGF-B的表达,减轻肾组织纤维化有关。更多还原

【Abstract】 Objective:To investigate the effect of Jiejufushenning on renal protein kinase C(PKC),renal vascular endothelial growth factor(VEGF),renal platelet-derived growth factor B(PDGF-B),and renal protection in diabetic nephropathy(DN) rats. Methods:The diabetic SD rats induced by STZ were divided in to four groups:diabetic control group,Jiejufushenning group,Irbesartan group,and Jiejufushenning combined with Irbesartan group,and the normal control group as well.After the experimental rats in every group were respectively treated for 12 weeks by above intervention methods,the relative kidney weight,blood sugar,urea nitrogen(Bun),serum creatinine(Scr),and urinary albumin excretion rate(UAER) were detected by routine analysis methods,and PKC and VEGF in renal tissue were detected by immunohistochemical techniques,and PDGF-B in renal tissue was detected by Western blotting,and ultramicrostructure of kidney was observed by transmission electron microscope.Results:The expression of renal cortex PKC and VEGF was increased in diabetic rats.The ultramicrostructure of kidney was noticeably changed,and the blood sugar,UAER,BUN,Scr,relative kidney weight,and PDGF-B were significantly increased in diabetic rats.While the above indexes in Jiejufushenning group,Irbesartan group,and Jiejufushenning combined with Irbesartan group were significantly lower than those in diabetic control group(P<0.05),the ultramicro- structure of kidney changed was improved,and the improvement of all indexes in Jiejufushenning combined with Irbesartan group was the most obvious(P<0.05).Conclusion: Themechanism of the protection of Jiejufushenning on renal lesion may be decreasing the level of PDGF-Bin the renal tissue and down regulating the expression of PKC and VEGF.更多还原