【作者】 杨清； 姜孝芳； 王宏伟； 司红莉； 高丽；

【Author】 GAO Li YANG Qing JIANG Xiao-Fang WANG Hong-wei SI Hong-li Basic Medical School of Xin Jiang Medical College,The Xinjiang Medical college sixth affiliated hospital Urumqi 830011,China

【机构】 新疆医科大学第六附属医院； 新疆医科大学；

【摘要】 目的:探讨辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠的血清中白介素-5(Interleukin-5,IL-5)、白介素-13(Interleukin-13,IL-13)和鼻粘膜组织中的Eotaxin、CCR3、信号转导和转录激活因子-6(signal transducers and activator s of transcription-6,STAT6)mRNA表达,探讨其可能药效作用机制。方法:40只大鼠随机分为空白对照组、模型组、对照组、实验组,用卵清蛋白建立AR大鼠模型,通过对造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用酶联免疫吸附技术(Enzyme labeled immunosorbent assay,ELISA)定量测定各组大鼠血清IL-5、IL-13水平及用原位杂交(ISH)方法半定量检测鼻粘膜中Eotaxin、CCR3、STAT6mRNA的阳性表达。结果:实验组大鼠血清的IL-5、IL-13表达水平和鼻粘膜中STAT6mRNA阳性表达比例明显低于模型组及阳性对照组(P<0.05)。结论:辛芷鼻敏胶囊可能降低STAT6mRNA阳性表达,抑制大鼠AR发病过程中IL-5,IL-13的表达水平,进而减少嗜酸性粒细胞浸润而控制症状。更多还原

【Abstract】 Objective:To explore the effect ofXinzhi Bimin capsule on the content of IL-5、IL-13 in blood serum and the express of Eotaxin、CCR3、STAT6 mRNA in nasal mucosa of rat models with allergic rhinitis and identify the mechanism of pharmacodynamic action.Methods:Forty rats were divided into four groups at random as normal control group,model group,positive control group and experimental group.Ovalburnin sensitized rats used as animal of AR were taken orally with Xinzhi Bimin capsule and compared with the different model rat established in AR and the normal control group, the expression of positive cell of STAT6 mRNA in nasal mucosa was detected in semi quantity by in situ hybridization(ISH) and the expression of IL-13 in blood serum was detected in quantity by ELISA; Results:The expression of the proportion of positive cell to Eotaxin、CCR3、STAT6 mRNA in nasal mucosa and the level of IL-5、IL-13 in blood serum were markedly lower in the experimental group than the model group(P<0.05) and the positive control group(P<0.05) except the normal control group. Conclusion:The result indicates that the capsule of Xinzhi Bimin can reduce the expression of Eotaxin、CCR3、STAT6 mRNA in nasal mucosa of rats and down-regulate the content of IL-5、IL-13 in sera,whereby to treat AR in rats.更多还原