【作者】 裴强； 赵习德；

【Author】 PEI Qiang,ZHAO Xi-de~*,LIU Shou-hua. * The First Affiliation Hospital of Xin-xiang Medical College,Weihui 453100,China

【机构】 河南省卫辉市新乡医学院第一附属医院中西医结合科；

【摘要】 目的:观察桂枝茯苓胶囊对动脉粥样硬化（AS）模型大鼠的影响。方法:SD大鼠随机分为空白组12只和造模组38只。空白组予普通饲料;造模组予高脂饲料+维生素D₃腹腔注射,造模成功后将造模组随机分为模型组、西药对照组、治疗组各12只。分别予生理盐水、辛伐他汀合卡托普利、桂枝茯苓胶囊灌胃,16周末处死动物。测定血清ET-1、NO、hs-CRP含量及主动脉细胞间粘附分子-1（ICAM-1）、基质金属蛋白酶9（MMP-9）、金属蛋白酶1组织抑制剂（TIMP-1）的表达。结果:与空白组相比,模型组ET-1、hs-CRP、ICAM-1、MMP-9积分光密度明显增高,NO明显减低（P<0.05）;与模型组比较,治疗组与西药对照组ET-1、hs-CRP、ICAM-1、MMP-9积分光密度均明显降低,NO、TIMP-1积分光密度明显升高（P<0.05）,两组ET-1与空白组相比差异无统计学意义（P>0.05）;治疗组NO高于西药对照组,两组血清ET-1、hs-CRP、ICAM-1、MMP-9、TIMP-1积分光密度相比差异无统计学意义（P>0.05）。结论:桂枝茯苓胶囊具有抗AS的作用。更多还原

【Abstract】 Objective:To observe the anti-atherosclerosis effects of Guizhi Fuling Capsule.Methods: 50 healthy SD male rats were randomly divided into 2 groups:normal group（n=12）,AS model group（n=38）.The model of experimental AS rats was established by high cholesterol diet and intraperitoneal injecting VitD₃.The normal control group was fed by ordinary diet.AS model group was randomly divided into 3 groups:AS group（n=12）,control group（n=12）,therapy group（n=12）.therapy group were given Guizhi Fuling Capsule by intragastric administration.Control group were given simvastatin and captopril by intragastric administration.The AS group were given partes aequales 0.9%NaCl instead of Guizhi Fuling Capsule.The rats were killed at the 16th weekend and aorta arch and breast aorta were observed.Blood fat and serum levels of Endothelin-1（ET-1）,Nitric oxide（NO）, high-sensitivity C-reactive protein（hs-CRP） were detected respectively.Immunohistochemistry technology was used for observing the expression of intercellular adhesion molecule-1（ICAM-1）,matrix metalloproteinase-9（MMP-9） and Tissue-Inhibitor of Metalloproteinase-1（TIMP-1） in breast aorta. Results:The levels of ET-1,hs-CRP and the amount of ICAM-1,MMP-9 expression in AS group were higher and the level of NO were lower than that in normal group（P<0.05）.Compared with AS group,the levels of ET-1,hs-CRP and the amount of ICAM-1,MMP-9 expression in therapy group and control group decreased obviously,the level of NO and the amount of TIMP-1 increased obviously（P<0.05）.The levels of ET-1 did not have significant difference among the groups of therapy,control and normal（P>0.05 ）.the level of NO in therapy group were higher than that in control group（P<0.05）.The levels of ET-1,hs-CRP and the amount of ICAM-1,MMP-9,TIMP-1 expression did not have significant difference between therapy group and control group.Conclusions:Guizhi Fuling Capsule has the effects of Anti-atherosclerosis to rats with AS.更多还原