【作者】 杨慧； 曲光刚； 赵元楷； 王金良； 沈志强；

【Author】 YANG Hui~1,QU Guang-gang~2,ZHAO Yuan-kai~2,WANG Jin-liang~(1,2),SHEN Zhi-qiang~(1,2*) (1 shandong LvDu Bio-sciences Technology Co.,Ltd.Binzhou 256600; 2 Shandong Binzhou Animal Sciences & Veterinary Medicine Academy.Binzhou 256600)

【机构】 山东绿都生物科技有限公司； 山东省滨州畜牧兽医研究院；

【摘要】 为提高犬细小病毒（CPV）的检出率,降低检测成本,本研究采用环介导等温扩增（LAMP）技术,建立了一种新型敏感、特异、快速、简便、实用的可视化检测犬细小病毒（CPV）的技术。针对CPV VP2基因保守区设计6条引物,进行LAMP扩增,对扩增反应进行优化,并检测其敏感性、特异性。所建立的方法最佳反应时间为60min,具有良好的特异性,与同属的其它病毒无交叉反应,本方法对CPV的最低检出量为9.3×10^-5ng/μL,其敏感性是PCR的100倍。该方法与胶体金试纸法的符合率达100%。结果表明,该方法特异性强、敏感性高,并且操作简便、检测成本低,检测时间短,无需特殊仪器,在CPV的快速诊断方面具有很高的实用价值。更多还原

【Abstract】 A Loop-mediated isothermal amplification(LAMP)method was developed for new kind,specific,rapid,simple and sensitive detection of canine parvovirus(CPV).A set of six primers were designed based on the CPV VP2 gene sequence to recognize the canine parvovirus(CPV).The LAMP reaction mix was optimized.The most optimal reaction time of the LAMP method for the CPV gene were 60min.The sensitivity test indicated that the LAMP was 100 times higher than that of PCR.These results suggested that detection of canine parvovirus by LAMP is an effective and low-cost procedure with high specificity and sensitivity that requires no specialized equipment.Without special equipment,in terms of rapid diagnosis of CPV are highly practical use.更多还原