【作者】 刘洁； 夏兴霞； 王永山； 张雪寒； 费荣梅； 何孔旺；

【Author】 LIU jie~(1,2),XIA Xing-xia~1,WANG Yong-shan~1,ZHANG Xue-han~1,FEI Rong-mei~2,HE Kong-wang~(1*) (1.Institute of Veterinary Medicine,Jiangsu Academy of Agricutural Sciences/ Key Laboratory of Animal Diseases Diagonostic and Immunology,Ministry of Agriculture/ National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014 China;2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)

【机构】 江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心； 南京农业大学动物医学院；

【摘要】 用颗粒为20nm的胶体金溶液标记纯化的羊抗鼠IgG,制备金标探针,喷涂于玻璃纤维膜上;将大肠杆菌O157:H7抗原和抗大肠杆菌O157:H7单克隆抗体F1分别包被于硝酸纤维素膜上作为检测线和质控线,组装成鼠血清O157:H7抗体胶体金免疫层析检测试纸条。实验结果:该试纸条仅与O157:H7阳性鼠血清发生特异性反应;用该试纸条与常规间接ELISA平行比较检测O157:H7免疫鼠血清,抗体效价分别为10~6和10~5,两者的敏感度相当。该试纸条操作简便,10min可出结果,适用于调查与追溯鼠O157:H7感染状况时的现场检测。更多还原

【Abstract】 To develop a rapid and accurate test based on the principle of gold immunochromatographic assay for the detection of antibodies against enterohemorrhagic E.coli O157:H7 in mice,the size of 20nm gold particles in colloidal solution was coupled with purified goat anti-mouse IgG,which was called gold probes,and was dispensed on the glass fibers.The EHEC O157:H7 antigen and mouse anti-EHEC O157:H7 monoclonal antibody F1 were separately coated on the nitrocellulose membrane as the test line(T line) and the control line(C line).The rapid gold immunochromatographic (IC) strip was assembled in regular sequence.The results indicated that the positive serum produced a positive signal on the IC strip,whereas the negative one did not.The O157:H7 positive serum was comparatively detected with both IC and indirect ELISA,and the antibody titers were 10~6 and 10~5 respectively.The IC strip developed in this study is specific, simple,sensitive,and suitable for the field investigation of EHEC O157:H7 infection condition in mice.更多还原