【作者】 刘长彬； 倪建宏； 张宾； 卢守亮； 万鹏程；

【Author】 LIU chang-bin,NI jian-hong~*,ZHANG bin,LU Shou-liang,WAN Peng-cheng Xinjiang Academy of Agricultral and Reclamation Sciences,shihezi 832000,China

【机构】 新疆农垦科学院畜牧兽医研究所；

【摘要】 本研究着重探讨和研究奶牛体外受精(in vitro fertilization,IVF)各技术环节中一些因素对奶牛IVF整个过程的影响,初步建立一套比较完善的操作技术体系。其结果如下:IVF实验:实验一,不同来源的卵母细胞对奶牛IVF的影响:分别对采用屠宰场采集的卵母细胞和活体采卵收集的卵母细胞进行体外培养,受精后36h和7d后观察卵裂率和囊胚发育率,差异不显著。说明卵母细胞来源对IVF的影响不显著。实验二:对精子分别采用percoll梯度密度离心和上浮法处理,两组的卵裂率没有明显差异,囊胚发育率虽有所提高,但差异不显著。实验三,不同培养体系对奶牛IVF的影响:分别用SOF液和BO液作为体外培养系统。两者培养体系的卵裂率和囊胚发育率均差异不显著。实验四,受精时间分别采用8h、10h、12h。三组的卵裂率逐渐降低,但差异不显著。囊胚发育率受精时间8h、10h及10h、12h均差异不显著,但受精8h的囊胚发育率显著高于受精时间12h。实验五,受精微滴(100μl)中放置成熟的卵母细胞数分别为20、40个,两组的卵裂率和囊胚发育率均下降,并且差异显著。更多还原

【Abstract】 The aim of this study was to establish an steady and efficient(in vitro fertilization,IVF) system in our lab.Experiments were carried out to analyze the different factors reported influence the results of IVF in dairy cattle.Study of IVF:1.IVF with oocytes from OPU and slaughterhouse.The results showed that cleavage rates and blastocyst rates were not significantly different between the two groups.2.The sperm were treated by percoll and upriver.There were no significant differences at cleavage rates and blastocys rates between the two groups.3.The effects of different culture systems(SOF、BO) on IVF of dairy cattle.The cleavage rates and blastocys rates in SOF were not significantly different between the two groups.4.Cleavage rates were respectively at the fertilization time 8h、10Oh and 12h.There were no significant differences between the three groups.The blastocyst rates at 8h were higher than atl2h。But the blastocyst rates were not significantly different between 8h and 10h and between 10h and 12h.5.The different oocytes number added to fertilization dripping(20、40).The results showed that the former cleavage rates and blastocyst rates was higher than the latter.更多还原