【作者】 丁家波； 杨宏军； 康孟佼； 程君生； 毛开荣； 蒋玉文；

【Author】 DING Jia-bo~1,YANG Hong-jun~2,RANG Meng-jiao~1,CHENG Jun-sheng~1, MAO Kai-rong~1,JIANG Yu-wen~1 (1 China Institute of Veterinary Drug Control,Beijing 100081,China; 2 The center of dairy cow,Shandong Academy of Agricultural Sciences,Jinan 200031,China)

【机构】 中国兽医药品监察所； 山东省农业科学院奶牛研究中心；

【摘要】 为了探索布鲁氏菌在豚鼠和奶牛体内所引起的抗体水平和变态反应强度的相关性,分别用1×104CFU和3×104CFU牛种布鲁氏菌强毒2308株各感染12只豚鼠,30天后,测定抗体滴度和变态反应强度。感染后第35天,扑杀豚鼠,取脾脏测定每克脾脏含菌量。用布鲁氏菌A19疫苗,以5×1010CFU皮下注射布病阴性黑白花奶牛50头,分别于免疫后15天、30天、60天测定抗体滴度,并于免疫后45天测定变态反应强度。运用SPSS 17.0-Analyze-Correlate-Bivariate Correlations程序分析试验数据,结果显示:不同剂量布鲁氏菌2308感染豚鼠后30天所诱导的抗体水平、变态反应强度和克脾脏含菌量三者之间均无相关性。A19疫苗免疫奶牛后60天的抗体水平与免疫45天的变态反应强度呈正相关,免疫15天和30天的抗体水平和变态反应强度无相关性。豚鼠试验结果表明,抗体水平、变态反应强度与个体对布病的抵抗力均无相关性。更多还原

【Abstract】 To investigate the correlations between the antibody level and allergic response induced by B.abortus 2308 and vaccine strain A19,two doses(1×104CFU and 3×104CFU )of 2308 were infected to 12 guinea pigs,respectively.Thirty days post infection(DPI),the antibodies were titrated by micro-agglutination test(MAT),and the allergic response was measured as well next day.The spleen cfu of bacteria per-gram was counted 35 DPI.Fifty dairy cows aged between 3 and 6 months were inoculated subcutaneously with dose of 5×1010 CFU of A19,and the antibodies were titrated at 15 DPI,30 DPI and 60DPI,respectively,while the allergic response was measured 45 DPI.The above data about the antibodies and allergic response were analysized with SPSS 17.0(Analyze - Correlate - Bivariate Correlations),and it shows that there is no correlation among the antibody level,allergic response degree and the spleen cfu of bacteria per-gram induced by 2308 in giunea pigs.The positive correlation could only be found between the antibody level 60DPI and allergic response degree 45DPI induced by A19 in dairy cows.From the guinea pigs test,It could concluded that no matter the antibody level nor allergic response degree correlates with the resistance to brucella.更多还原