【作者】 成子强； 刘思当； 张利； 赵振华；

【Author】 CHENG Zi - qiang~1,LIU Si - dang~1,ZHANG Li~1,ZHAO Zhen - hua~2 (1.Animal Science and Technology College,Shandong Agricultural University,Tai’an271018,China; 2.Animal Science and Medicine College,Inner Mongolia Agricultural University,Huhhot010018,China)

【机构】 山东农业大学动物科技学院； 内蒙古农业大学动物科学与医学学院；

【摘要】 本研究通过原位杂交(ISH)和免疫组织化学(IHC)技术检测了组织中ALV-J病毒RNA的表达及定位。原位杂交结果显示:在攻毒后3周时,所检测到的组织大部分已感染病毒;病毒对肝脏、心脏、肾脏的实质细胞、骨髓髓系细胞和卵巢基质中的间质细胞、脾脏红髓内的单核-巨噬细胞有较高的嗜性,在核膜及胞浆内显示出蓝紫色颗粒状的特异性信号,而在法氏囊、胸腺、脑和坐骨神经中检测不到病毒RNA及病毒基因的表达。免疫组化结果与原位杂交相似,在核膜及胞浆内可见蓝紫色阳性信号,瘤组织的信号较强,显示了较强的抗原性。由骨髓和其他组织的结果可推测ALV-J诱导肿瘤可能和病毒基因的插入位点有直接关系,而和病毒在组织内的数量没有直接关系。更多还原

【Abstract】 Avian myelcytomatosis is induced by avian leukosis virus subgroup J(ALV -J) in broiler chick - ens.In this paper an in situ hybridization(ISH) technique and immunohitochemistry technique were usedto detect RNA transcripts and locat the antigen in tissues from chickens infected with ALV - J Inner Mongo - lia strains(NM8761).2 chickens were killed at 3,5,7,8,10,14,23,27 weeks respectively,different tissueswere collected for further tests.The results of in situ hybridization and immunohistochemistry were identi -cal.The greatest env expression was observed in cells specific to the tumor,liver,heart,kidney,myeloidlinear cells,overy parenchymal cells.Viral gene expression could not be detected in the bursa of Fabri -cius,thymus,brain,ischiadic nerve in both tests.All the results indicated that myelocytes neoplasia hadcorrelation with insertion site of ALV - J provirus in host cells genome,and had not direct correlation withthe volumes of virus.更多还原