【作者】 张志； 崔治中； 姜世金；

【Author】 ZHANG Zhi,CUI Zhi-zhong~*,JIANG Shi-jin (College of Animal Science and Technology,ShandongAgricultural University,Taian,Shandong271018,China)

【机构】 山东农业大学动物科技学院；

【摘要】 将表现为典型J亚群禽白血病肿瘤的病料分别接种于鸡胚成纤维细胞和DF1细胞,采用间接荧光抗体试验(IFA)和PCR方法,从这些肿瘤病料中分离和鉴定出8株J亚群禽白血病病毒(ALV-J)。对证明感染了ALV-J的细胞继续培养,并用禽网状内皮组织增生症病毒(REV)的特异单抗及引物做IFA和PCR,在8株ALV-J中,有3株ALV-J的感染细胞中同时有REV感染。由此表明,发生肿瘤的肉鸡中,ALV-J和REV的共感染已相当普遍。将这3株REV-SD0003、SD0004和SD0102的3’-LTR用PCR扩增、克隆和序列比较,发现分离的SD0003、SD0004和SD0102与国内的另外2株REV-SD9901和HA9901的同源性为96.7%和96.1%;而与另1株REV参考株鸭脾坏死性病毒(SNV)的同源性高达99%。更多还原

【Abstract】 This study was performed on chicken embryo fibroblast cells(CEF) and DF1 cell inoculated with tumor samples.After culturing for 7 days,we isolated and identified 8 strains of subgroup J avian leukosis virus(ALV - J) by indirect fluorescence antibody assay(IFA) and polymerase chain reaction(PCR).The cells that infected ALV -J were further cultured and detected by monoclonal antibody and primers speific to reticuloendotheliosis virus(REV) in IFA and PCR.Among the 8 ALV - J strains,3 were co - infected with REV.The results suggested that dual infection of ALV - J and REV seems common in meat - type chickens.Amplification,clone and comparison of Long Terminal Region from REV - SD0003,SD0004 and SD0102 were implied that the sequence of our REV showed 92%,96%and 99%homology as compared with REV - SD9901 and HA9901 strains isolated before in China and another reference strain of SNV.更多还原