【作者】 彭运平； 齐维； 唐海波； 刘晓云；

【Author】 PENG Yun-ping~(1,2),QI Wei~1,TANG Hai-bo~1,LIU Xiao-yun~(1,2) (1.Guangzhou Wondfo Biotech Co.,Ltd,Guangzhou 510530,China)(2.College of Light Industry and Food Sciences of South China University of Technology,Guangzhou 510641,China)

【机构】 广州万孚生物技术有限公司； 华南理工大学轻工与食品学院；

【摘要】 目的:检测鱼肉、蜂蜜中氯霉素的残留,以评价试剂盒性能。方法:采用间接竞争酶联免疫分析法,通过标准曲线和线性范围、检测限、准确度和精密度、交叉反应等分析试剂盒的适用性。结果:标准曲线的R²为0.9943,线性方程y=0.3668x+0.4353,IC₅₀为0.71μg/L,线性范围为0.05～4.05ng/rmL。鱼肉和蜂蜜中的检测限分别为0.126μg/kg和0.081μg/kg。鱼肉和蜂蜜的添加回收率分别大于88.1%和85.5%,批内变异系数分别小于15.8%、13.6%,批间变异系数小于15.5%、12.3%。与氯霉素琥珀酸钠的交叉反应率为152.63%,与其他药物的交叉反应率均小于0.01%。结论:该法测定氯霉素灵敏准确,重复性好,特异性高,适合用于检测鱼肉、蜂蜜中的氯霉素残留。更多还原

【Abstract】 Objective To detect the residues of chloramphenicol in fish and honey,and assess the kit’s properties.Methods An indirect competitive enzyme-linked immunosorbent assay（ELISA） was used to analyze the kit’s applicability through standard curve and linearity range,detection limit, accuracy and precision,cross reaction,etc.Results The R² of standard curve was 0.9943,and the linear equation was y=0.3668x+0.4353,IC₅₀=0.71μg/L. Quantitation of chloramphenicol was linear from 0.05ng/ml to 4.05ng/ml.The limit of detection was 0.126μg/kg in fish and 0.081μg/kg in honey.The recovery rates in fish meat and honey were more than 88.1%and 85.5%,inter-assay coefficient variations were below 15.8%,and 13.6%,and intra-assay coefficient variations were below 15.5%and 12.3%.The cross-reactivity towards chloramphenicol succinate sodium was 152.63%,while below 0.01% towards other drugs.Conclusion This method could meet the needs of detecting the residues of chloramphenicol in fish and honey with satisfactory sensitivity,accuracy,repeatability and specificity.更多还原