【作者】 覃小丽； 王永华； 杨博； 黄惠华；

【Author】 QIN Xiao-li~1,WANG Yong-hua~1,YANG Bo~2,HUANG Hui-hua~1 (1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China) (2.School of Bioscience and Biotechnology,South China University of Technology,Guangzhou 510006,China)

【机构】 华南理工大学轻工与食品学院； 华南理工大学生物科学与工程学院；

【摘要】 本文以低附加值的猪油为原料高效制备富含1,3-二油酸-2-棕榈酸甘油三酯的结构脂,该结构脂将应用于母乳脂替代品的开发。以Lipozyme RM IM酶催化酸解低度分提液体猪板油与油茶籽油脂肪酸的反应制备富含1,3-二油酸-2-棕榈酸甘油三酯的结构脂;通过气相色谱检测目标产物的总脂肪酸和Sn-2位脂肪酸组成。结果表明,最优条件为34℃猪板油与油茶籽油脂肪酸摩尔比为1:4,加酶量为底物质量的6%,反应温度45℃,反应时间6 h,油酸的结合率高达51.46%,目标产物的熔点为2.2℃。该制备方法的建立为母乳脂替代品的开发具有重要意义。更多还原

【Abstract】 Enzymatic acidolysis synthesis of triacylglycerols rich in l,3-dioleoyl-2-palmitoylglycerol(OPO) was studied with lard and oil-tea camellia seed oil as substrates.The total fatty acid and sn-2 positional fatty acid of structured lipids rich OPO were determined by gas chromatography.Results showed that a high proportion of oleic acid to total fatty acids(51.46%) was obtained at reaction parameters with temperature of 45℃,6%of Lipozyme RM IM(wt%),a mole ratio 1:4 and reaction time 6 h.The melting point of triacylglycerols rich in OPO displayed main peak at 2.2℃.An efficient method was developed for the synthesis triacylglycerols rich in OPO,which can be used as infants’ dietary supplement.更多还原