【作者】 李瑞雪； 汪泰初； 胡飞； 贾鸿英； 樊美珍； 王伟；

【Author】 Li Ruixue1 Wang Taichu1 Hufei3 Jia Hongying1 Fan Meizhen2* Wangwei1 (1 The Sericultural Research Institute,AnHui Academy of Agricultural Sciences,Hefei 230061; 2 Anhui Agricultural University,Hefei 230036;3 The Plantprotection Research Institute,Hefei)

【机构】 安徽省农业科学院蚕桑研究所； 安徽省农业科学院植保研究所； 安徽农业大学；

【摘要】 通过比较不同提取方法及设计正交试验确定了蝉拟青霉中虫草素的最佳提取工艺:采取管式离心机离心发酵醪,收集菌丝体50℃热空气烘干,按菌粉:石油醚=1:1的量用石油醚脱脂,用布氏漏斗抽滤收集菌丝体烘干,最后用75%乙醇、45℃超声波浸提虫草素,提取4次,每次提取40min,料液比为1:20。更多还原

【Abstract】 According to the extraction effects of different solvents,the physical and chemical characters tests of cordycepin,the optimal extraction condition was determined as follows:(1) centrifuging the fermenting matter with centrifugal machine and collecting the mycelium to dry by hot air at 50 ℃.(2) degreasing the fungus powder with petroleum ether according to fungus powder:the benzine = 1:1’s quantity.(3) using 75% ethyl alcohol,45℃ supersonic extracting cordycepin,and the extraction run four times with the raw material-water ratio at 1/20 and it lasted 40 minutes each time.更多还原