【作者】 刘景福； 李恪梅； 佘菲菲； 王国治；

【Author】 LIU jing-fu1] LI ke-mei2] SHE fei-fei1] Wang guo-zhi2]1],Department of Pathogenic Biology Fujian Medical University,Fuzhou 350004,China;2],National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China

【机构】 福建医科大学基础医学院病原生物学系； 中国药品生物制品检定所细菌一室；

【摘要】 目的:通过XTT(四唑鎓盐)法检测布氏菌疫苗的活菌含量,探讨XTT法快速检测布氏菌活菌含量的可行性。方法:将XTT法运用于布氏菌疫苗活菌含量的检测,通过已知活菌浓度的布氏菌液,制备XTT法的吸光值与布氏疫苗活菌含量的关系参比曲线,并根据制备的参比曲线同样运用XTT法检测未知浓度的104M菌液的活菌量,比较传统CFU(活菌集落计数)活菌计数方法与XTT法两者检测布氏菌活菌含量的相关性。结果:XTT法检测布氏菌疫苗的最佳时间应在4h左右,在一定范围内XTT法所测得的吸光值与细菌活菌含量有较好的相关性,相关系数R2=0.9974。运用10支布鲁氏菌疫苗,通过XTT法与CFU法检测其活菌含量,两者结果相关性较好,Spearman相关系数为0.879,p=0.001。结论:XTT法能够在一定活菌浓度范围反应布氏菌疫苗活菌含量,可为快速检测布氏菌活菌含量提供一种新的参考方法。更多还原

【Abstract】 Objective To measure the live bacteria content of Brucella vaccine by XTT assay, and discuss the possibility that XTT would be a quick and simple method of detecting live bacteria content of Brucella vaccine. Methods XTT method was employed for Brucella vaccine .A linear regression was made between the absorbance of XTT and different concentrations of standard Brucella vaccine. The linear equation was used to read out the viable count of unknown samples by absorbance. Then the results were compared with the conventional viable count of the same samples. Results The optimum time of detecting Brucella vaccine was about 4 hours. The regression coefficient R2 might reach 0.9974.The data of viable count of 10 lots of Brucella vaccine by the XTT assay and colony forming unit (CFU) demonstrated a positive correlation with correlation coefficient, r = 0.879,p=0.001.Conclusions The method XTT could show different concentrations of Brucella vaccine in a certain range. The assay can be applied for rapid determination of viable count of Brucella vaccine.更多还原