【作者】 刘金花； 李佳； 张永清；

【Author】 LIU Jin-hua,LI Jia,ZHANG Yong-qing (Shandong University of Traditional Chinese Medicine,Jinan 250355)

【机构】 山东中医药大学；

【摘要】 目的研究不同温度(15℃,20℃,25℃,30℃和35℃)对黄芩种子发芽特性和次生代谢的调节。方法超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。结果黄芩种子的发芽率、发芽势和发芽指数均受到低温和高温的影响。黄芩种子最适宜的发芽温度为20-25℃;与适温相比,在低温和高温下SOD,POD和CAT的活性明显降低,PAL和C4H也呈现相类似的变化趋势,这与其次生代谢物质的变化也相同;次生代谢物质(黄酮类化合物)与其代谢途径中的关键酶(PAL和C4H)具有良好的相关性(r=0.956,r=0.951,p<0.05)。结论黄芩种子发芽以及次生代谢物质合成的适宜温度为20℃。黄芩药材中次生代谢物质的含量可通过提高PAL和C4H的活性来提高。更多还原

【Abstract】 OBJECTIVE To study the germination characteristics and secondary metabolism of Scutellaria baicalensis Georgi seeds under different temperature(15 ℃ , 20 ℃ , 25 ℃ , 30℃ and 35℃ ) . METHODS The activities of SOD and POD were determined with lactoflavine-NBT method and guaiacol-colorimetry assay, respectively. The CAT, APX,PAL and C4H were determined with ultraviolet spectrophotometry. The secondary metabolites were detected byHigh Performance Liquid Chromatography (HPLC). RESULTS The germination percentage, germination potentiality and germination index were seriously affected by low or high temperature. The proper germination temperature is 20-25 ℃ . The activity of superoxide dismutase (SOD), peroxidase(POD) and catalase (CAT) were significantly decreased as comparison to suitable temperature. The activity of phenylalanine ammonia-lyase (PAL) and cinnamic acid 4-Hydroxylase (C4H) were also significantly decreased as comparision to suitable temperature which is accordance to the secondary metabolites. There is a positive correlation between the flavonoids content and the PAL and C4H activity(r=0.956,r=0.951, p<0.05). CONCLUSION The quality of the skullcaps can be improved by improving the activity of PAL and C4H. The proper temperature for the seed germination and the formation of secondary metabolites is 20 ℃ .更多还原