【作者】 陈霡沁； 周燕； 谭文松；

【Author】 Chen mai qin,ZHOU Yan and TAN Wen-SongState Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)

【机构】 华东理工大学生物反应器工程国家重点实验室；

【摘要】 采用各种方法制备具有良好生理活性的模块组织,模拟体内组织构建方式体外构建仿生组织是目前的新兴的研究方向。本研究将细胞与CuitispherS大孔微载体共培养形成装配所需单位模块组织,再利用灌注塑型体外构建大型骨组织。结果表明经培养28d后微构组织具有良好细胞活性及丰富的胞外基质,灌注塑型可得达厘米级结构(13mm×10mm)的大组织仍具有成骨表型。实验结果表明,采用微组织体外构建工程化大型骨组织可为骨组织构建提供新的研究方向。更多还原

【Abstract】 With the desire to create more complex tissues with features such as developed and functional microvasculature,cell binding motifs and tissue specific morphology,tissue engineering techniques are beginning to focus on building modular microtissues with repeated functional units.Here we investigated the feasibility of this modular tissue system for the in-vitro maturation of Cultispher S microcarriers seeded with hAMSC to generate macro-bone tissue.Compact macrotissue can be assembled from microtissue,with homogenous ECM distribution and osteogenic differentiation,and maintained high cellular viability in the macrotissue,with a centimetre-sized(12mm×6mm).The results shows that the modular tissue system could be assamble by perfusion,and it would provid a novel idea to construct engineered tissue ex vivo.更多还原