【作者】 朱雅男； 邹颖； 王春雨； 郭世梁； 张凯； 王文梅；

【Author】 Zhu Yanan,Zou Ying,Wang Chunyu,Guo Shiliang,Wang Wenmei(Department of dental and endodontics,Institute of Stomatology Nanjing University Medical School,Nanjing 210008,China).

【机构】 南京大学口腔医学院口腔内科；

【摘要】 目的:通过对四种粘固材料的体外仿生矿化,观察和检测材料表面元素沉积、晶体形态和组分,比较其生物活性,为选择完全性纵折后牙的粘结材料提供实验基础。方法:将普通型玻璃离子、加强型玻璃离子、SuperBond C&B、SE BOND和光固化型氢氧化钙(对照)分别制成直径5mm、高2mm圆片形试件。将试件分别置入含10ml模拟体液的64孔细胞培养板,37℃静置孵育14天。扫描电镜(scanning electron microscope,SEM)观察表面形貌。X射线能谱(X-ray energy dispersive spectroscopy,EDS)检测样品表面化学元素组成。傅立叶转换红外光谱仪(Fourier transform infrared spectroscopy,FTIR)分析化合物基团。结果:SEM结果显示,氢氧化钙和SuperBond表面光洁有球状晶体聚集,前者直径约为1-1.5um,后者约为2-5um;玻璃离子表面粗糙,普通型表面可见直径约为5-10m不规则晶体,加强型表面为球形小晶体(1-1.5um);SE BOND表面呈现玻璃熔融状,晶体形状消失,表面有碎屑样物沉积。EDS元素分析显示,氢氧化钙和玻璃离子及SuperBond表面有大量钙、磷沉积,钙磷比值达3.28、9.04和1.87(接近羟基磷灰石(HA)1.53)。FTIR检测结果显示,存在羟基(3431 cm-1、1639cm-1),PO43-(1037cm-1、561cm-1)及不完全CO32-(1451cm-1、1413cm-1或871cm-1)的吸收峰,和HA的振动峰相同且有CO32-进入磷灰石晶格结构中。结论:在适宜的条件下SuperBondC&B相对具有较好的生物学潜力和活性。更多还原

【Abstract】 Objective:To observe the elements deposition and crystals morphology on the cements surface after biomimetic mineralization in order to evaluate the biological activity of the materials and to select the best cement for reparation of complete vertical fractured teeth.Methods:Wafer-shaped specimens with 5mm diameter,2mm height were made of glass ionomer,reinforced glass ionomer,SuperBond C&B,SE BOND and light curing calcium hydroxide(control).Specimens were mineralized in simulated body fluid for 14 days.The morphology,composition and phase of mineral grown on PLGA were analyzed with Scanning electron microscope(SEM),Fourier transform infrared spectroscopy(FTIR) and X-ray energy dispersive spectroscopy(X-EDS).Results:SEM results showed that on calcium hydroxide and SuperBond smooth surface with spherical crystal aggregation was observed.The diameter of the former was about 1 -1.5um and the other was about 2-5um. The surface of glass ionomer is rough.On the surface of ordinary glass ionomer irregular crystals of 5-10um were showed.While on the surface of enhanced glass ionomer small spherical crystals(1-1.5um) were aggregated.SE BOND surface displayed of glass melted.The shape of crystal disappeared but debris deposition was observed.EDS results showed that calcium and phosphorus deposited on the surface of calcium hydroxide, glass ionomer and SuperBond,the ratios of calcium and phosphorus were 3.28,9.04 and 1.87(close to hydroxyapatite(HA) 1.53).FTIR results show that there is absorption peaks of hydroxyl(3431 cm-1、1639cm-1),P043-(1037cm-1、561cm-1) and incomplete C032-(1451cm-1、1413cm-1 or 871cm-1).So it is with HA and CO32- went into the apatite lattice structure.Conclusion:Apatite may produce and grew by glass ionomer and SuperBond on its surface under the proper conditions.SuperBond showed a better biological potential and activity.更多还原