【作者】 刘源； 杨燃； 李继遥； 周学东；

【Author】 Liu Yuan,Yang Ran,Li Jiyao,Zhou Xuedong(West China College of Stomatology, Sichuan University)

【机构】 四川大学华西口腔医学院；

【摘要】 目的:本研究通过植物凝集素ConA对口腔细菌凝集、粘附和生物膜形成作用的研究,为进一步发现控制牙菌斑生物膜的有效措施、寻找药物作用的新靶点、开拓龋病预防的新措施奠定实验基础。方法:使用植物凝集素ConA对口腔致龋菌和常驻菌在不同浓度、不同pH和不同糖作用下进行凝集反应;评估植物凝集素ConA对细菌粘附的作用;利用激光共聚焦显微镜结合特异荧光染料标记口腔细菌生物膜的方法观察刀豆凝集素作用下细菌生物膜形态、结构的变化。结果:在中性环境下,一定浓度ConA可以引起变异链球菌、表兄链球菌、血链球菌的凝集,D-葡萄糖、D-甘露糖可以抑制ConA引起的凝集反应。经过Co nA处理SHA,变异链球菌和表兄链球菌在其上的粘附明显被抑制,其中ConA对表兄链球菌的作用更为明显。ConA处理细菌后,并没有引起粘附的抑制,在低浓度阶段,随着浓度的提高,粘附量也呈上升趋势。Co nA对S.mutans ATCC 251 75生物膜的形成和厚度均与常态生物膜没有显著差异。结论:通过研究发现不同浓度的ConA可以引起变异链球菌、表兄链球菌和血链球菌产生凝集现象,同时可以此三种细菌对唾液获得性膜的粘附。Co nA能否发展成为预防龋病和抗粘附治疗的药物,还有待进一步研究。更多还原

【Abstract】 Purpose:This work aimed to evaluate the potential effects of plant lectin ConA on the aggregation and adherence and bioflim formation of Streptococcus mutans in vitro.Methods:The aggregation was performed with different concentrations of ConA at different pH and with different kinds of sugar.The adherence inhibition was performed on saliva-coated hydroxyapatite at which lectin solutions were previously incubated with S.mutans or with SHA.CLSM was used to observe ConA treated or untreated S.mutans bioflim formation.Results:1.ConA could aggregate S.mutans at high concentration and neutral pH condition.2.ConA could inhibit the attachment of S.mutans to acquired pellicle. 3.ConA had no effect on the biofilm formation of S.mutans.Conclusions:ConA was able to aggregate and inhibit the adherence of the S.mutans tested.更多还原