A novel technique for isolating orchid mycorrhizal fungi

【作者】 朱国胜； 喻子牛；

【Author】 Zhu,G.S.~(1,2,3),Yu,Z.N.~1,Gui,Y.~(1,2,3) and Liu,Z.Y.~(1,4*) 1 State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan,Hubei, 430070,PR China 2 Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang,Guizhou,550006,PR China 3 Institute of Biotechnology,Guizhou Academy of Agricultural Science,Guiyang,Guizhou,550006,PR China 4 Guizhou Academy of Agricultural Science,Guiyang,Guizhou,550006,PR China

【机构】 State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University； Guizhou Key Laboratory of Agricultural Biotechnology； Institute ofBiotechnology,Guizhou Academy of Agricultural Science；

【摘要】 <正>We describe a technique for isolating mycorrhizal fungi from roots of orchids.This technique involves selection and treatment of roots,preparation of pelotons,treatment of pelotons,culture of pelotons so that fungal hyphae grow out and strain purification.The technique is considered better because 1) problems of fungal and bacterial contamination are resolved,2) endophytic bacteria are suppressed and also used to promote hyphal growth from the pelotons,3) live and dead pelotons,and those from which fungi are culturable or unculturable can easily be identified,providing increased isolation efficiency,4) a single taxon can be isolated from a single peloton containing several mycorrhizal taxa,5) slow-growing mycorrhizal taxa can easily be isolated.The implications and potential use of this technique in future studies is discussed.更多还原

【Abstract】 We describe a technique for isolating mycorrhizal fungi from roots of orchids.This technique involves selection and treatment of roots,preparation of pelotons,treatment of pelotons,culture of pelotons so that fungal hyphae grow out and strain purification.The technique is considered better because 1) problems of fungal and bacterial contamination are resolved,2) endophytic bacteria are suppressed and also used to promote hyphal growth from the pelotons,3) live and dead pelotons,and those from which fungi are culturable or unculturable can easily be identified,providing increased isolation efficiency,4) a single taxon can be isolated from a single peloton containing several mycorrhizal taxa,5) slow-growing mycorrhizal taxa can easily be isolated.The implications and potential use of this technique in future studies is discussed.更多还原