【作者】 柏建玲； 吴清平； 张菊梅； 莫树平； 胡文杰； 胡事君；

【Author】 BAI Jian-Ling~1 WU Qing-Ping~(1**) ZHANG Ju-Mei~1 MO Shu-Ping~1 HU Wen-Jie~1 HU Shi-Jun~2 (1 Guangdong Institute of Microbiology;Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application;Guangdong Open Laboratory of Applied Microbiology,Guangzhou,China,510070;2 Dongguan reseach center of agricultural science,Dongguan,Guangdong,China,523000)

【机构】 广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室； 东莞市农业科学研究中心；

【摘要】 利用本实验室分离保存的乳酸菌菌种17株,经过耐胃液试验筛选,得到耐胃液能力较强、生长良好的乳酸菌菌株5株,选取4株菌株利用API乳酸菌生化系统和16SrDNA分析鉴定,并进行耐胃液驯化选育。结果表明,鉴定的4株乳酸菌中2株(编号:BR1-2和R8)为副干酪乳杆菌(Lactobacillus paracasei),1株(编号:BR3-2)屎肠球菌(Enterococcus faecium),1株(编号:BR2-2)乳酸片球菌(Pediococcus acidilactici);驯化后各菌株耐胃液能力均有不同程度的提高,其中BR1-2经人工胃液处理存活率提高52.2%;其他三株菌株的存活率也均提高20%以上。更多还原

【Abstract】 By gastric resistant test,5 gastric tolerable strains were screened from 17 strains of the conserved strains.To identify 4 strains of the 5 with API Lactic acid bacteria bio-chemistry system and 16SrDNA analyses,and then to domesticate their gastric tolerable property.The result showed that the 2 of the 4 identified strains(BR1-2 and R8) are Lactobacillus paracasei.Another two strains,one is Enterococcus faecium, the last one is Pediococcus acidilactici.After the domestication,the gastric resistant ability of the 4 strains is higher than before.The survival rate of BR1-2 was advanced 52.2%,the other 3 strains ’survival rates were advanced over 20%.更多还原