【作者】 朱弘焱； 苏玉虹； 于百平； 宋恒元；

【Author】 SU Yu-hong,ZHU Hong-yan,YU Bai-ping,SONG Heng-yuan (Liaoning Medical University Animal Husbandry and Veterinary institutee,Liaoning,Jinzhou 121000,China)

【机构】 辽宁医学院畜牧兽医学院； 沈阳正成原种猪场； 辽宁省畜牧科学院；

【摘要】 [目的]分析辽宁种猪群体Mx1基因多态性与免疫性能之间的关系。[方法]提取猪耳组织DNA,采用PCR-RFLP技术分析荷包猪、大白猪的Mx1基因多态性,采用双抗体夹心法酶联免疫分析(ELISA)方法分析两个种群中IFN-α、IL-4、SIgA的水平。[结果]PCR-RFLP研究后发现,大白猪在该位点为单态,但荷包猪存在AA,AB,BB三种基因型,基因型频率为0.6814、0.0619、0.2566;荷包猪群体免疫数据整体高于大白猪。[结论]荷包猪与大白猪Mx1基因频率存在差别,荷包猪群体免疫水平高于大白猪,且携带更多Mx1基因的抗病性基因型;本研究为辽宁种猪资源保存、品种选育和开发利用提供了科学依据。更多还原

【Abstract】 [Objective]To analyze the Mxl gene polymorphism of Liaoning Province pigs and its relationship with immune traits.[Method]To extract DNA from ear in pigs,the technology of Hin6 I PCR-RFLP was used,and use ELISA to analyze the levels of IFN-α,IL-4,SIgA.[Result]Mx1 gene digested by PCR-RFLP only in Hebao pigs was divided into three kinds of genotypes of AA,AB and BB,the frequencies of genotypes were 0.6814,0.0619 and 0.2566;the ELISA data of Hebao pigs were higher than Large White as a whole.[Conclusion]The immunity was higher in Hebao pigs than in Large White,and had much more resistance genotypes of Mx1;the research provided the scientific basis for the resource preservation,variety breeding and the development and utilization of Hebao pigs.更多还原