【作者】 边慧慧； 黄倢； 王秀华； 蒋继志；

【Author】 BIAN Hui-Hui~(1,2)HUANG Jie~(1,2*)WANG Xiu-Hua~1 JIANG Ji-Zhi~3 (1 Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao,266071) (2 ShangHai Ocean University,ShangHai,201306)(3 Hebei University,Baoding,071002)

【机构】 农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所； 上海海洋大学； 河北大学；

【摘要】 用添加绿色荧光蛋白(GFP)饲料投喂牙鲆,研究了不同消化时间内食糜在牙鲆消化道内的分布情况,采用福林-酚法测定了不同时间胃、前肠、中肠、后肠各组织粘液蛋白酶活性。结果表明:牙鲆在摄食后消化道蛋白酶活性随着食糜在消化道内的分布而变化,食物的刺激引起消化腺体分泌蛋白酶。牙鲆摄食后1h食糜达到中肠,胃和中肠蛋白酶活性与空腹相比迅速增高;食糜在5h时遍布整个消化道,此时胃蛋白酶活性几乎检测不出,而前肠蛋白酶活性迅速增高,5h达到最高,然后开始降低;8h时中肠蛋白酶活性一直很高,之后降低;而后肠直到12h其蛋白酶活性仍维持在较高水平,说明牙鲆这种消化道较短的鱼类其肠道比胃在蛋白质消化过程中发挥更重要的作用。该结果在海水鱼类口服药物研发方面可以作为参考依据。更多还原

【Abstract】 Adding green fluorescent protein(GFP) to the bait,the distribution of the chyme in the digestive tract of flounder Paralichthys olivaceus was observed.The protease activity in the mucus of the digestive tract was studied by the Folin-phenol reagent method post ingestion.The results showed that the protease activity changed with chyme distribution in the digestive tract after ingesting.The chyme reached the midgut 1h after ingesting,the protease activity of stomach and midgut increased rapidly.The chyme distributed in the whole digestive tract after 5h,the stomachic protease activity reduced to near null.The protease activity in the midgut kept at the highest level until 8h.The protease activity in the hindgue kept at the high level until 12h after ingesting. The results show that the intestines might be more significant in protein digestion than the stomach for the fish with short digestive tract.These results might be used as reference for the oral administrated drugs in fish.更多还原