【作者】 赵鹏伟； 曹贵方； 梁建荣；

【Author】 ZHAO Peng-wei,CAO Gui-fang~*,LIANG Jian-rong (College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)

【机构】 内蒙古农业大学动物科学与医学学院；

【摘要】 绵羊β-防御素(SBD-1)与T载体成功连接,并将其成熟肽编码片段亚克隆到了毕赤酵母表达载体Ppic9k上,构建了真核表达质粒Ppic9k-mSBD-1。且将其在毕赤酵母GS115中进行了分泌表达。将表达产物纯化后进行了活性鉴定。结果表明,mSBD-1对大肠杆菌和金黄色葡萄球菌均有抑制作用,而且对金黄色葡萄球菌的抑制作用更为明显。更多还原

【Abstract】 the ovis β-defensin(SBD-1)was linked with the T vector.And the region of coding mature peptide was subcloned with the Ppic9k(Pichia pastoris expression vector). The recombination vector Ppic9k-mSBD-1 was constructed.The mSBD-1 was secreted in GS115. And the secretion was purified and the activity was determined.The result showed that mSBD-1 can inhibit the growth of the Escherichia coli and Staphylococcus aureus,and the inhibition was significantly on Staphylococcus aureus.更多还原